بررسی ساختار شیمیایی کلاژن ماهی سفید (Rutilus Kutum) از دریای خزر و کلاژن ماهی قباد Scomberomorus guttatus) ( از خلیج فارس

نوع مقاله : علوم جانوری

نویسندگان

1 گروه زیست شناسی دریا، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

2 سازمان تحقیقات آموزش وترویج کشاورزی، موسسه تحقیقات علوم شیلاتی کشور، تهران، ایران

3 گروه جراحی پلاستیک و ترمیمی، مرکز تحقیقات سوختگی، دانشگاه علوم پزشکی ایران، بیمارستان حضرت فاطمه (س)، تهران، ایران

4 گروه فارماکولوژی، دانشکده علوم پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران

5 گروه میکروبیولوژی کاربردی، دانشگاه علوم پزشکی بقیه الله، تهران، ایران

چکیده

کلاژن دارای کاربردهای فراوانی در زمینه داروسازی و پزشکی، تهیه محصولات بهداشتی و آرایشی و صنایع غذایی می­باشد. در سال­های اخیر توجه زیادی به جداسازی کلاژن از موجودات دریایی شده است که علت آن عدم محدودیت استفاده از آن در رژیم غذایی و عدم ایجاد خطر ابتلا به بیماری­های مسری می­باشد. در این مطالعه به استخراج کلاژن ازپوست ماهی سفید از ماهیان اقتصادی دریای خزر و ماهی قباد یکی از گونه­های تن ماهیان خلیج فارس پرداخته شده است. کلاژن  پوست ماهی سفید و ماهی قباد به­روش اسید و باز استخراج گردید و سپس به­وسیله  Pico.Tag ، SDS-PAGE، FTIR و اسپکتوفوتوگرافی UV مورد ارزیابی قرار گرفتند. نتایج براساسSDS-PAGE  و ترکیبات اسیدهای آمینه نشان دادند که کلاژن پوست هر دو ماهی کلاژن نوع I  و از دو زنجیره α (1α و2α) با وزن مولکولی kDa 130 و مقدار جرِئی β تشکیل شده است. هم­چنین تجزیه و تحلیل FTIR، ترتیبات مارپیچی از هر دو کلاژن را نشان داد و اسپکتروفتومتری UV در هر دو نمونه کلاژن پوست ماهی سفید و ماهی قباد حداکثر جذب را در 240 نانومتر نشان داد. میزان کلاژن استخراج شده از پوست ماهی سفید 15/6% و ماهی قباد 14/5% بود. تجزیه و تحلیل اسیدآمینه در کلاژن­های استخراج شده، مقدار بالایی از گلایسین را در هر دو نمونه کلاژن، ماهی سفید 182/5 residues بر 706/1 residues و ماهی قباد 188/6 residues  بر 706/1 residues را نشان داد که آن یک سوم از کل اسیدهای آمینه محاسبه گردید و هم­چنین مقدار پرولین به­عنوان یک اسیدآمینه منحصر به­فرد در کلاژن ماهی سفید 89/6 residues  بر 706/1 residues و در ماهی قباد residues  86/8 بر 706/1 residues بود.

کلیدواژه‌ها


عنوان مقاله [English]

The chemical structure of acid-soluble Collagen from the Skin of Rutilus Kutum of Caspian Sea and Scomberomorus guttatus of Persian Gulf

نویسندگان [English]

  • Soheila Naderi QaraqeshlaQ 1
  • Shahla Jamili 2
  • Mohammad Javad Fatemi 3
  • Ali Mohammad Sharifi 4
  • Mohammad Reza Nourani 5
1 Department of Marine Biology, Science and Research Branch, Islamic Azad university, Tehran, iran.
2 Agricultural Research and Development Organization, Fisheries Research Institute of Iran, Tehran, Iran
3 Plastic & amp; Reconstructive Surgery Department, Burn Research Center, Tehran, Iran.
4 pharmacology Department , Iran University of Medical Sciences, Tehran, Iran
5 Biotechnology Department , Baqiyatallah University of Medical Sciences
چکیده [English]

Collagen has a wide range of applications in biomedical, pharmaceutical, cosmetic, and food industries. Collagen from cow has a risk for contamination from bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE). This study proceeds to extract collagen from the skin of  Rutilus Kutum main and economical species of the Caspian Sea and Scomberomorus guttatus. Scomberomorus guttatus  one of the tuna species of Persian Golf. Acid-soluble collagens (ASC) from the fish skin of Scomberomorus guttatus and Rutilus Kutum were extracted and characterized by Pico.Tag, SDS-PAGE, FTIR and UV spectrophotometry. Based on the data from the SDS-PAGE and the composition of amino acid, both collagens  were probable that the obtained collagen classified as type I collagen. Collagens consisted of two different types of α chains, including α1 and  α2,and  β component. Also, FTIR analysis revealed the presence of helical arrangements of collagens. Moreover, UV spectrophotometry exhibited this collagen have an absorbance at 220 nm. According to the basis of dry weight, the yield of Rutilus Kutum  and Scomberomorus guttatus ASC were calculated 15.6 % and 14.5 %. Amino acid analysis revealed a much higher glaycin content in the both collagens extracted which accounted to one third of the total amino acids. Besides, proline as an unique amino acid in Rutilus Kutum  and Scomberomorus guttatus ASC had the specific amount of89.6  and  86.8 residues per 706.1 residues.

کلیدواژه‌ها [English]

  • Rutilus Kutum
  • Scomberomorus guttatus
  • Fish skin Collagen
  • Characterization
  • Amino acid composition
  • Caspian Sea
  • Persian Gulf
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