مقایسه برخی پارامترهای ایمنی غیراختصاصی موکوس پوست گاوماهی زرد و گاوماهی خالدار

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 487-49175

چکیده

 تحقیق حاضر با هدف مقایسه پارامتر های ایمنی موکوس پوست گاوماهی زرد (Neogobius pallasi) و گاوماهی خالدار  (Neogobius melanostomus) انجام شد. از تعداد 10 عدد ماهی به­ترتیب 5 عدد از هر گونه نمونه­برداری موکوس صورت گرفته و فاکتورهای ایمنی غیراختصاصی شامل: پروتئین محلول، لیزوزیم، آلکالین فسفاتاز قلیایی و ایمنوگلوبولین کل موکوس دو گونه اندازه­گیری شد. پس از پایان آزمایش مشخص شد که میزان پروتئین محلول (به­ترتیب 0/22±3/14 و 0/32±1/23)، ایمنوگلوبولین کل (به­ترتیب 0/02±0/48 و 0/12±1/09)، آلکالین فسفاتاز (به­ترتیب 0/89±41/19 و 1/52±30/88) و مقدار لیزوزیم (به­ترتیب 1/00±10/60 و 0/67±8/87) بود. اختلاف معنی­داری بین سایر پارامترهای اندازه­گیری شده در دو گونه وجود داشت (0/05>P) و به­طورکلی میزان این پارامترها در گاوماهی زرد بالاتر از گاوماهی خالدار بود. در مجموع  با توجه به نتایج به­دست آمده می­توان گفت موکوس می­تواند نقش مهمی در ایمنی غیراختصاصی داشته باشد و تفاوت­های دو گونه در مورد واکنش به شرایط استرس­زا را می­توان تا حدودی به پارامترهای ایمنی غیراختصاصی موکوسی نسبت داد.

کلیدواژه‌ها

عنوان مقاله [English]

The comparison of some nonspecific immune parameters of skin mucus in Neogobius pallasi and Neogobius melanostomus

نویسندگان [English]

  • Marjan Hoseini
  • Hamed Kolangi
  • Hamed Azadi
  • Omid Amiri
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, PO Box: 487-49175
چکیده [English]

The present study aimed to compare nonspecific immune parameters and protein pattern of  skin mucus in Neogobius pallasi and Neogobius melanostomus. The mucus samples were collected from 10 specimens ( 5 of each species ). Total protein, alkaline phosphatase ( ALP ), lysozyme and total immunoglobulins of the samples were measured (according to the porotocol). The measured levels of non-specific immune parameters in Neogobius pallasi were, 3.14±0.22, 41.19±0.89, 10.60±1.00 and 0.48±0.02 and in Neogobius melanostomus were 1.23±0.32, 30.88±1.52, 8.78±0.67 and 1.09±0.12 respectively. Non-specific immune parameters that measured in all instances, were higher in Neogobius pallasi and there were significant differences between the two species. Therefore, results showed that skin mucus has an important role in innate immunity and two species differences in response to stressful situations can be partly attributed to mucosal immune parameters.

کلیدواژه‌ها [English]

  • Mucus
  • Lysozyme
  • Alkaline phosphatase
  • Immunoglobulin

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فصلنامه محیط زیست جانوری
دوره 10، شماره 3
مهر 1397
صفحه 265-270

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