مطالعه اثر پکتین به عنوان افزودنی غذایی در مقابله با مسمومیت کادمیمی در ماهی کپورمعمولی (Cyprinus carpio)

نوع مقاله : محیط زیست جانوری

نویسندگان

1 گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرم آباد، ایران

2 گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی خاتم الانبیاء، بهبهان، ایران

3 گروه شیلات، دانشکده منابع طبیعی، دانشگاه خلیج فارس، برازجان، ایران

چکیده

 این مطالعه به بررسی اثر حفاظتی پکتین بر تغییرات برخی فاکتورهای بیوشیمیایی خون ماهی کپور معمولی (Cyprinus carpio) در مواجهه با کادمیم پرداخته است. برای این منظور غلظت‌های مختلف زیرکشنده کادمیم (125 و 250 میلی­گرم در کیلوگرم جیره خشک) به تنهایی و هم­چنین به­صورت ترکیبی با  0/5 درصد پکتین به جیره غذایی افزوده شد. بچه­ماهیان کپورمعمولی (میانگین وزنی 7±37/5 گرم) به­مدت 21 روز با شش جیره غذایی مختلف تغذیه شدند. ماهیانی گروه اول (شاهد) تنها با خوراک پایه بدون هیچ گونه افزودنی، گروه دوم و سوم به­ترتیب با 150 و 250 میلی­گرم کادمیم در کیلوگرم غذا، گروه چهارم و پنجم با 150 و 250 میلی­گرم کادمیم به­همراه 0/5 درصد پکتین و گروه ششم تنها با 0/5 درصد پکتین تغذیه شدند. نمونه‌گیری از خون ماهیان پس از 21 روز تغذیه با جیره­های غذایی اختصاصی هر گروه صورت گرفت و متعاقباً برخی از پارامترهای بیوشیمیایی خون مورد سنجش قرارگرفتند. مطابق با نتایج، درهر دو گروه دریافت ­کننده کادمیم و پکتین میزان آنزیم‌های AST، ALT و ALP، پروتئین کل، آلبومین، گلبولین، تری­گلیسرید، کراتینین و گلوکز تغییرات معنی‌داری نسبت به گروه شاهد نشان ندادند. در گروه‌های دریافت­ کننده کادمیم به تنهایی سطح آنزیم­های AST و ALT تغییرات معنی‌داری نسبت به گروه شاهد نشان داد. بر مبنای نتایج به­دست آمده به­نظر می رسد که پکتین به شکل بالقوه­ای می­تواند اثرات مضر ناشی از فلزات سنگین را کاهش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Study on the effects of pectin, a feed additive against cadmium toxicity in common carp (Cyprinus carpio)

نویسندگان [English]

  • Mohammad Mohiseni 1
  • Mohammad Amini Chermahini 2
  • Maryam Karimi 2
  • Dara Bagheri 3
1 Department of Fisheries, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran
2 Department of Fisheries, Faculty of Natural Resources, Behbahan, Khatam Alanbia University of Technology, Behbahan, Iran
3 Department of Fisheries, Natural Resources Faculty, University of the Persian Gulf, Borazjan, Iran
چکیده [English]

This study was investigated the protective effects of pectin on changes in some blood biochemical parameters against cadmium exposure in common carp. Fish juveniles (37.5±7 g) were fed by 6 different diets during 21 days. The first group (control) was only fed by commercial diet. The diets for second and third group were prepared by addition sublethal dosage of 150 and 250 mg cadmium (per kg diet), respectively. For the fourth and fifth groups, the commercial diet was supplemented by 150 or 250 mg cadmium/kg diet plus 0.5 percent of pectin. Similar level of pectin has been used as a unique feed additive for the last group. Following 21 days feeding trial, blood sampling was withdrawn separately for all six mentioned groups and some plasma biochemical parameters were analyzed, subsequently. Plasma levels of AST, ALT, ALP, total protein, albumin, globulin, triglyceride and creatinine were remained undisturbed in pectin plus cadmium groups compared to control (P>0.05). On the other hands, the most above factors were changed in only cadmium administrated groups compared to the control. Based on obtained results, it seems that pectin potentially can ameliorate the harmful effects of heavy metals.
 

کلیدواژه‌ها [English]

  • Heavy metal
  • Biosorption
  • Common carp
  • Blood biochemistry
  • Pectin
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