تاثیر منابع مختلف کربن بر جمعیت باکتریایی و مشخصات بافت سنجی روده کپورمعمولی (Cyprinus carpio) درسیستم بیوفلاک

نوع مقاله: محیط زیست جانوری

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران

2 گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

در­این تحقیق، تاثیر منابع مختلف کربن (ملاس چغندر و پودر تفاله هویج) بر تعداد کل باکتری ­ها و تغییرات بافتی روده ماهی کپورمعمولی (Cyprinus carpio) در سیستم بیوفلاک مورد بررسی قرار گرفت. تعداد 243 قطعه ماهی با میانگین وزنی (8/1±16/2)گرم تهیه و با تراکم 9 عدد در هر آکواریوم50 لیتری به­ مدت 8 هفته تیمار شدند. در­مجموع 9 تیمار آزمایشی شامل تیمار1 (بدون سیستم بیوفلاک با 100% جیره روزانه) و 8 تیمار در سیستم بیوفلاک شامل تیمار 2 (100%)، تیمار 3 (75%)، تیمار 4 (50%) و تیمار 5 (25%) جیره روزانه + منبع کربنی پودر تفاله هویج و تیمار 6 (100%)، تیمار 7 (75%)، تیمار 8 (50%)، تیمار 9 (25%) جیره روزانه + منبع کربنی ملاس چغندر­قند مورد ارزیابی قرار گرفتند.در ­پایان آزمایش  جمعیت باکتریایی روده و محیط پرورشی و نیز مشخصات بافت­ سنجی بررسی شدند. نتایج به ­دست آمده نشان داد که تفاوت معنی ­داری (0/05>p) در تعداد باکتری­ های روده و هم­ چنین آب محیط پرورش بین تیمار­های بیوفلاک و تیمار شاهد وجود دارد به ­طوری که بیش ­ترین تعداد باکتری­ های روده در تیمار 5 (LogCFU/g 7/7±0/008) و کم ­ترین آن (LogCFU/g 6/08±0/018) در تیمار شاهد است و بیش­ ترین تعداد باکتری­ های محیط پرورش در تیمار 6(LogCFU/ml 6/18±0/023)و کم ­ترین در تیمار شاهد(LogCFU/ ml 4/18±0/03) به ­دست آمد. افزایش معنی ­دار (0/05>p) طول پرز­های روده در تیمار 3 مشاهده شد. استفاده از سیستم بیوفلاک و افزودن منبع کربنی سبب تغییر در جمعیت کل باکتری­ های آب محیط پرورش،روده و تغییراتی از ­جمله افزایش طول پرز، سلول­ هایجامی وسلول­ های لوکوسیت دربافت روده گردید.

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