تاثیر منابع مختلف کربن بر جمعیت باکتریایی و مشخصات بافت سنجی روده کپورمعمولی (Cyprinus carpio) درسیستم بیوفلاک

نوع مقاله : محیط زیست جانوری

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران

2 گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

در­این تحقیق، تاثیر منابع مختلف کربن (ملاس چغندر و پودر تفاله هویج) بر تعداد کل باکتری ­ها و تغییرات بافتی روده ماهی کپورمعمولی (Cyprinus carpio) در سیستم بیوفلاک مورد بررسی قرار گرفت. تعداد 243 قطعه ماهی با میانگین وزنی (8/1±16/2)گرم تهیه و با تراکم 9 عدد در هر آکواریوم50 لیتری به­ مدت 8 هفته تیمار شدند. در­مجموع 9 تیمار آزمایشی شامل تیمار1 (بدون سیستم بیوفلاک با 100% جیره روزانه) و 8 تیمار در سیستم بیوفلاک شامل تیمار 2 (100%)، تیمار 3 (75%)، تیمار 4 (50%) و تیمار 5 (25%) جیره روزانه + منبع کربنی پودر تفاله هویج و تیمار 6 (100%)، تیمار 7 (75%)، تیمار 8 (50%)، تیمار 9 (25%) جیره روزانه + منبع کربنی ملاس چغندر­قند مورد ارزیابی قرار گرفتند.در ­پایان آزمایش  جمعیت باکتریایی روده و محیط پرورشی و نیز مشخصات بافت­ سنجی بررسی شدند. نتایج به ­دست آمده نشان داد که تفاوت معنی ­داری (0/05>p) در تعداد باکتری­ های روده و هم­ چنین آب محیط پرورش بین تیمار­های بیوفلاک و تیمار شاهد وجود دارد به ­طوری که بیش ­ترین تعداد باکتری­ های روده در تیمار 5 (LogCFU/g 7/7±0/008) و کم ­ترین آن (LogCFU/g 6/08±0/018) در تیمار شاهد است و بیش­ ترین تعداد باکتری­ های محیط پرورش در تیمار 6(LogCFU/ml 6/18±0/023)و کم ­ترین در تیمار شاهد(LogCFU/ ml 4/18±0/03) به ­دست آمد. افزایش معنی ­دار (0/05>p) طول پرز­های روده در تیمار 3 مشاهده شد. استفاده از سیستم بیوفلاک و افزودن منبع کربنی سبب تغییر در جمعیت کل باکتری­ های آب محیط پرورش،روده و تغییراتی از ­جمله افزایش طول پرز، سلول­ هایجامی وسلول­ های لوکوسیت دربافت روده گردید.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of different carbon sources on bacterial count and histometric of Cyprinus carpio intestine, reared in a biofloc system

نویسندگان [English]

  • Saeed Asadollah Nasrabadi 1
  • Preeta Kochanian 1
  • Nasorllah Mahboobi Soofiani 2
  • Vahid Yavari 1
  • Amir Hossein Jallali 2
1 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 Department of Fisheries, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

In this study, effect of different carbon sources (carrot pomace and molasses) on total bacterial count and histology of intestine in Cyprinus carpio, reared in a biofloc system was investigated. 243 fish (16.8±2.1g BW) were purchased and randomly distributed in 50-liter aquarium (9/aquarium) for 8­weeks. Nine different treatments, including a treatment with 100 % daily feeding rate (3%­BW) with water exchange as a control, the other 8­treatments were assigned to biofloc systems, including treatment 2­(100%), 3­(75%), 4­(50%) and 5­(25%) daily feeding rate in addition to carbon source carrot pomace and 4 other treatments, including treatment 6­(100 %), 7­(75%), 8­(50%) and 9­(25%) daily feeding rate in addition to carbon source molasses. At the end of experiment, total number of bacteria in the aquaria water and intestine of each fish was counted. Additionally, intestine histology was investigated using H&E. The results showed that there are significant differences in bactria counts of intestine and water between biofloc treatments and control. The highest number of intestinal bacteria (7.7±0.008 logCFU/g) was related to treatment­5 and the least of that was observed in control (6.08±0.018 logCFU/g). The highest number of bacteria in water media (6.18±0.023 logCFU /ml) was obtained in treatment6 and the least of that was related to the control (4.18±0.03 logCFU/ml). Significant (p<0.05) increase in villi length was observed in treatment­3. The biofloc system and the addition of carbon source caused changes in bacterial count of intestine and water culture media besides alterations in villi length, club shaped cells and leukocytes of carp intestinal.

کلیدواژه‌ها [English]

  • Intestinal histometry
  • Bacterial count
  • Cyprinus carpio
  • Biofloc technology

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فصلنامه محیط زیست جانوری
دوره 10، شماره 4
دی 1397
صفحه 351-360

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