Effects of dietary probiotic, Lactococcus lactis “subspecies PTCC 1403” on the growth parameters and survival rate of grey mullet (Mugil cephalus L.) against Lactococcus garvieae bacteria

Document Type : Nutrition

Authors

1 Department of Fisheries, Marine Sciences Faculty, Chabahar Maritime University, Chabahar, Iran

2 Department of Fisheries, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran, PO Box: 83111-84156

Abstract

The present study was conducted to investigate the effects of diet supplementation  with probiotic Lactococcus lactis “subspecies PTCC 1403” on the growth performance and survival rate of grey mullet (Mugil cephalus) for 30 days. The experiment was conducted in a completely randomized design with 450 grey mullet juveniles with average weight of 8.44±1.40g in 5 treatments and 3 replicates (n=30 in each replicate), consisting of two control treatments using non-supplemented food, and three experimental treatments (D1, D2 and D3) which were supplemented with this probiotic with concentrations of 1.5× 108, 3×108, 6×108  CFU /g feed respectively. After 30 days feeding with experimental diets, 10 fish per replication of experimental treatments and one control treatment were challenged by immersion method withlive pathogenic bacteria Lactococcus garvieae (1×107 CFUml-1) for 1 minute and their mortality were recorded for 10 days. The results showed that all of the measured growth parameters and survival rate in treatments containing  probiotic improved, but there were not significant differences compared to control group (P>0.05), and only the Condition Factor (CF) parameter in D2 and D3 treatments was significantly different with the control group (P<0.05). Survival percent increased to 64% in D3 and 68% in D2 compared with 40% in the control group (P <0.05). Overall we concluded that the using of this probiotic in the diet of grey mullet could increase survival rates against Lactococcus garvieae bacteria.

Keywords

References

  1. Ali, A., 2002. Probiotics in fish farming. Evaluation of a bacterial mixture PhD Thesis. University of Agricultural Sciences. Sweden.
  2. Burbank, D.R.; Shah, D.H.; LaPatra, S.E. and Fornshell, K.D., 2011. Enhanced resistance to coldwater disease following feeding of probiotic bacterial strains to rainbow trout. Aquaculture. Vol. 321, pp:185-190.
  3. Chen, S.; Suhko, S.; Wu, C.; Chaung, H.; Tasai, Y.; Yang, K.; Chen, Y.; Chen, T.; Lin, G.; Cheng, S.; Lin, Y.; Lee, J.; Lai, C.; Weng, Y. and Chu, Y., 2002. Lactococcus garvieae, a cause of disease in grey mullet (Mugil cephalus L.), in Taiwan.Journal of fish Diseases. Vol. 12, pp: 727-732.
  4. Choi, W.; Lam, C.L.; Mo, W.Y.; Cheng, Z.; Mak, N.K.; Bian, Z.X. and Wong,M.H., 2014. Effect of the modified Hunglian Jiedu decotion on the resistance in grey mullet (Mugil cephalus L.) to Lactococcus garvieae. Marine pollution Bulletin. Vol. 85, pp: 816-823.
  5. Doménech, A.; Prieta, J.; Fernández-Garayzábal, J.F.; Collins, M.D.; Jones, D. and Dominguez, L., 1993. Phenotypic and phylogenetic evidence for a close relationship between Lactococcus garvieae and Enterococcus seriolicida.Journal of Microbiology. Vol. 9, pp: 63-68.
  6. EFSA (European Food  Safety Authority). 2008. Technical  guidance  prepared  by  the Panel  Additives  and  Products  or Substances used in Animal Feed  (FEEDAP) on  the  update of the criteria  used in the assessment of bacterial resistance to antibiotics of human or veterinary importance.http://www.efsa.eu.int/cs/BlobServer/ScientificOpinion/feedapopej732tgantimicrobialresistanceen.pdf .
  7. Elder, A. and Ghittino, C., 1999. Lactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss similar, but different diseases. Diseases Aquatic Organisms. Vol. 36, pp: 227-231.
  8. Ferguson, R.M.W.; Merrifield, D.L.; Harper, G.M.; Rawling, M.D.; Mustafa, S.; Picchietti, J. L.; Balcazar, J. l. and Davies, S.J., 2010. The effect of  pediococcus acidilactici on the gut microbiota and immune status of on growing red tilapia. Journal of Applied Microbiology. Vol. 109, pp: 851-862.
  9. Fuller,  R., 1992.  History  and  development of  probiotics.  In:  Fuller,  R.  (Ed.), Probiotics: The Scientific Basis. Champan and Hall, New York. 108 p.
  10. Gatesoupe, A. and Mikkelson, H., 1999. Effect of supplementing the feed in turbot with  lactic acid bacteria and immono-stimulating peptides during a challenge trail with vibrio angullarium. Aquaculture. Vol. 167, pp: 103-113.
  11. Gildberg, A.; Mikkelsen, H.; Sandaker, E. and Ringo, E., 2002. Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of atlantic cod (Gadus morhua). Journal of Hydrobiologia. Vol. 352, pp: 279-285.
  12. Gomez-Gil, B.; Roque, A. and Turnbull, J.F., 2000. The use of a selection of probiotic bacteria for use  in  the  culture  of  larval aquatic organisms. Aquacul. Vol. 9, pp: 259-270
  13. Heo, W.S.; Kim, Y.; Kim, E.; Bai, S. and Kong, S., 2013. Effects of dietary probiotic, Lactococcus lactis subsp. lactis I2, supplementation on the growth and immune response of olive flounder. Aquaculture.Vol. 24, pp: 376-379.
  14. Jobron, A.; Olsson, J.C.; Westerdahl, A.; Conway, P.L. and Kejellberg, S., 1997.Colonization in the fish intestinal tract and production of inhibitory substance in intestinal mucus and faecal extract by carnobacterium sp strain k1. Fish diseases. Vol. 20, pp: 383-392.
  15. Kaoort, S.B.; Tucker, J.W.; Thoresen, M. and Sennett, D.G., 2004. Current methodology for the use of probiotic bacteria in the culture of marine fish larvae. World Aquaculture Society. Vol. 33, pp: 286-291.
  16. Lemos, M.L.; Dopazo, C.P.; Toranz, A.E. and Barja, L., 1995. Competitive dominance of antibiotic producing marine bacteria in mixed cultures. Journal of Applied Bacteriology. Vol. 71, pp: 228-232.
  17. Macey, B.M. and Coyne, V.E., 2005. Improved growth rate and disease resistance in farmed Haliotismidae through probiotic treatment. Aquaculture. Vol. 245, pp: 249-261.
  18. MacFarland, J.F., 2000. Biochemical testes for  identification of medical bacteria. Williams and Wilkins. New York.
  19. McIntosh, D.; Samocha, T.M.; Jones, E.R.; Lawrence, A.L.; McKee, D.A.; Horowitz, S. and Horowitz, A., 2000. The effect of a commercial bacterial supplement on the high-density culturing of Litopenaeus vannamei with protein diet in an outdoor tank system and no water exchange. Aquacultural Engineering. Vol. 21, pp: 215-227.
  20. Mocanu, M.; Cristea, V.; Dediu, L.; Bocioc, E.; Gerecu, R. and Vasilean., I., 2013.The effect of probiotic diet on growth and hematology parameters of rainbow trout. Agricultural Sciences and Veterinary Medicine Iasi. Vol. 59, pp: 258-263.
  21. Pirarat, N.; Pinpimai, K.; Endo, M.; Katagiri, T.; Ponpornpisit, A.; Chasue, N. and Maita, M., 2011. Modulation of intestinal morphology and immunity in Nile tilapia (Oreochromis niloticus)by Lactobacilus rhammnosus GG. Reserch in Veterinary science. Vol. 91, pp: 92-97.
  22. Vadstein, O.; Oie, G.; Olsen, Y.; Salvesen, I.; Skjermo, J. and Skjakbraek, K., 1993. A strategy to obtain microbial control during larval development of marine fish, Fish Farming Technology. Vol. 43, pp: 167-188.
  23. Vendrell, D.; Balca´zar, J.L.; Ruiz-Zarzuela, I.; de Blas, I.;Girone´s, O. and Muzqu´iz, J.L., 2006. Lactococcus garvieae in fish: A review. Comparative Immunology Microbiology and Infectious Diseases. Vol. 29, pp: 177-198.
  24. Yasuda, K. and Taga, N., 1980. A mass culture method for Artemia salina using bacteria as food. Journal of Microbial. Vol. 18, pp:53-62.
Journal of Animal Environment
Volume 10, Issue 4
December 2018
Pages 367-374

Files

Share

How to cite

Statistics