نوع مقاله : تنوع زیستی
نویسندگان
1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران
2 مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNACytochrome oxidase subunit I (COI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using PCR method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. The COXI sequence from 60 individuals with haplotype diversity was 0.47 and a nucleotide diversity was 0.0500. The analyzes with the BioEdit Composition procedure indicated that the sequence for COXI was 28.87% nucleotide A, 25.25% nucleotide C, 15.86% nucleotide G and 29.66% nucleotide T, which had a C + G ratio of 42% and A + T is 58%. Phylogenetic analysis of haplotype in the combination with the goat from Gen Bank showed that Iranian goat clustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.
کلیدواژهها [English]