مطالعه انگل های خونی در قورباغه مردابی Pelophylax ridibundus در شمال ایران

نوع مقاله: زیست شناسی (جانوری)

نویسندگان

گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

چکیده

 بی ­دمان گونه ­هایی از دوزیستان نیمه ­آبزی هستند که انگل­ های خونی مختلفی را متحمل می ­شوند. در این مطالعه در مجموع 66 قورباغه مردابی بالغ از شمال ایران بین اپریل و جولای سال 2017 به ­منظور بررسی فون انگل­ های خونی در آن­ ها گرفته شد. نمونه­ های خون با توجه به اندازه قورباغه از ورید صورت یا قلب گرفته شد. اسمیرهای خونی خشک شدند، با متانول فیکس شده و توسط گیمسا رنگ­ آمیزی شدند. نمونه ­ها توسط میکروسکوپ نوری با استفاده از بزرگ ­نمایی 100 و روغن ایمرسیون به­ منظور جستجوی انگل­ های خونی مورد آزمایش قرار گرفتند. فون انگل­ های خونی شامل ریکتزیاها و هموگری گارینی ­های داخل گلبول قرمز و میکروفیلاریا و اشکال مختلف تریپانوزوم در خارج گلبول قرمز خون مشاهده شد. این اولین مطالعه جامع مورفولوژیکی روی فون انگل­ های خونی قورباغه مردابی در ایران است، مطالعه­ ای که انتظار می ­رود استفاده از آنالیزهای مولکولی به ­منظور شناخت بهتر این انگل­ های خونی، ناقل­ های احتمالی و دینامیک سیکل زندگی آن­ ها را توسعه دهد.

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