اثر غلظت های تحت کشنده نانوذرات نقره و نیترات نقره بر ماهی کپورمعمولی (Cyprinus carpio. L): تغییرات خون شناسی و آنتی اکسیدانی

نوع مقاله: فیزیولوژی (جانوری)

نویسندگان

گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

با توجه به کاربرد بالای نقره و هم­ چنین احتمال در معرض گرفتن آبزیان، امکان در معرض قرارگیری این موجودات با این فلز زیاد است. لذا این مطالعه با هدف بررسی و مقایسه اثرات تحت حاد نانوذرات نقره و نیترات نقره بر بقاء، شاخص ­های مختلف خونی و هم­ چنین آنزیم ­های شاخص آنتی­ اکسیدانی در ماهی کپورمعمولی انجام شد. ابتدا غلظت کشندگی 50% و 96 ساعته برای دو شکل نقره به ­دست آمد و سپس ماهیان به ­مدت 21 روز در معرض %15 و 30% از غلظت حاد محاسبه شده (0/4 و 0/8 میلی­ گرم در لیتر نانوذرات و هم­ چنین 0/025 و 0/045 میلی­ گرم در لیتر نیترات نقره) قرار گرفتند. متوسط غلظت حاد 96 ساعته برای نانوذرات نقره و نیترات نقره به ­ترتیب 0/02±0/29 و 0/05±0/15 میلی­ گرم در لیتر بود. نتایج نشان ­دهنده اختلالات مختلفی در شاخص ­های خون­ شناسی ازجمله کاهش تعداد گلبول­ های قرمز و افزایش تعداد گلبول­ های سفید بود. هم ­چنین آنزیم SOD در اکثر تیمارهای درمعرض قرار گرفته در مقایسه با گروه شاهد القاء شد. این مطالعه نشان داد نیترات نقره در غلظت های پایین ­تری اثرات کشندگی خود را اعمال می­ کند و هر دو شکل نقره تقریباً به شکل یکسان باعث تغیییرات در فاکتورهای ایمنی ذاتی و شاخص ­های استرس اکسیداتیو در ماهیان درمعرض قرار گرفته شدند اما در آنزیم SOD این تغییرات در گروه­ های مواجه شده با نیترات نقره کمی بیش ­تر بود. 

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