بررسی آرایه شناسی جنس Paraschistura (در جنوب ایران) با استفاده از توالی ژن میتوکندریایی (COI)

نوع مقاله : سیستماتیک جانوری

نویسندگان

1 گروه محیط زیست دریایی، دانشکده محیط زیست، کرج، ایران

2 گروه شیلات، پردیس کشاورزی و منابع طبیعی کرج، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

بررسی  تنوع و ساختار ژنتیکی گونه ­های مختلف ماهیان از اساسی ­ترین شاخص ­های لازم برای حفظ و بازسازی ذخایر آن­ ها است. این مطالعه بر آن است تا با استفاده ازروش ­های مولکولی با استفاده از توالی‏­ های به‌ ­دست ‌آمده از ژن سیتوکرم ­اکسیداز COI به بررسی آرایه ­شناسی سگ­ ماهی جویباری جنس Paraschistura در جنوب ایران بپردازد. به این منظور نمونه بالغ از هر گونه که شامل:7عددگونه  P. bamporensis، سه عدد گونه P. sargadensis، چهار عدد گونه P. hormozensis، و پنج عدد گونه Parascshistura sp.، از حوضه های جنوبی ایران جمع ­آوری گردید، DNA نمونه­ ها با استفاده از کیت ژنومی و پرایمر FCOI20ʹ استخراج گردیدو طیفرآیند PCR تکثیرو پس از الکتروفورز توالی­ یابی انجام گرفت. جهت بررسی روابط فیلوژنی توالی ­های ژن COI از نرم ­افزار MEGA6 استفاده گردید. نتایج این بررسی نشان داد که نمونه ­های جنس   Paraschistura در چهار کلاد قرار گرفته ­اند. نمونه ­های P. bamporensis از حوضه ­های مختلف با فاصله تکاملی (0/128-0/005) به ­همراه P. sargadensis و Parascshistura sp از حوضه سرباز در یک کلاد قرار گرفته ­اند. نمونه ­های  P. hormozensis با دارا بودن فاصله تکاملی (0/042)، در کلاد مجزایی قرار دارند. به ­همین ترتیب P. abdolii ،P. naumani و P. delvarii و با دارا بودن فواصل تکاملی (0/185-0/030) و نمونه Parascshistura sp. با فاصله تکاملی (0/206-0/042) در کلادهای مجزایی قرار گرفته ­اند. با توجه به فاصله حوضه ­ها از هم و بررسی خصوصیات مورفولوژی، نتایج مولکولی با نتایج مرفولوژیکی موجود مطابقت داشت. تجزیه و تحلیل درخت فیلوژنی در این مطالعه نشان می ­دهد ویژگی­ های ریخت­ شناسی مورفولوژیک به ­همراه شناسایی مولکولی برای تایید تشخیص گونه‌ها ضروری می‌ باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of Molecular taxonomic genus Paraschistura (in south of Iran) By using mitochondrial gene Sequences of (COI)

نویسندگان [English]

  • Mona Moghadam 1
  • Mohammadreza Rahmani 1
  • Soheil Eigderi 2
  • Samira Kiyany harchegany 1
1 Department of Marine Environment, Faculty of Environment, Karaj, Iran
2 Department of Fisheries, Faculty of Agriculture and Natural Resources of Karaj, Faculty of Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Study of the diversity and genetic structure of various fish species are one of the most basic indexes for preservation and restoration of their reserves. the aim of this study is to review taxonomy of a genus of hillstream loaches paraschistura, by molecular methods using gene sequences obtained by cytochrome oxidase gen in the south of Iran. For this purpose, an adult sample of any species which include: 7 specimens P.bamporensis, 3specimens P. sargadensis, 4specimens P.hormozensis, and 5specimens Parascshistura sp. was collected from south of Iran. DNA of these samples was extracted by genomic kit and FCOI20' primer, and was multiplied during PCR process and sequencing after electrophoresis. in order to study phylogenetic relations of COI gene of above mentioned fishes MEGA6 software was used .the results of the study showed that samples of paraschistura genus are placed in 4 clades. Samples of p.bampurensis from different locations with evolutionary distance (0.005-0.128) and P. sargadensis are located in one clade close to each other. samples of p.hormozensis with evolutionary distances (0.042) are located on separate clades. accordingly P. abdolii, P. naumani AND P. delvarii with evolutionary distances (0.042-0.206) and Parascshistura sp With an evolutionary distance(0.030-0.185) are located on separate clades.Considering the distance of location from each other and also study of morphological traits, molecular results were consistent with morphological traits. Phylogenetic tree analysis in this study shows that morphologic characters together with molecular identification are needed to support recognition of species.

کلیدواژه‌ها [English]

  • Phylogeny
  • Paraschistura
  • taxonomy
  • Genetic structure
  • Loach

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فصلنامه محیط زیست جانوری
دوره 11، شماره 2
مرداد 1398
صفحه 305-312

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