تاثیر غلظت های تحت کشنده دیازینون بر بیان ژن ویتلوژنین در جنس ماده ماهی گورخری (Danio rerio)

نوع مقاله : علوم جانوری

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

در این تحقیق تأثیر دوزهای مختلف سم دیازینون بر بیان ژن‌ یتلوژنین (Vtg) درجنس ماده ماهی گورخری (Danio rerio) بررسی شد. تعداد 600 قطعه بچه‌ ماهی‌ گورخری با میانگین وزنی 0/01±0/15 ‌گرم در 4 تیمار و 3 تکرار به ­مدت یک ماه تحت تاثیر سه دوز سم 0/8، 1/6 و 3/2 میلی­ گرم بر لیتر و تیمار شاهد قرار گرفتند. در انتهای دوره جهت مطالعات ژنتیکی از کبد نمونه‌ برداری و استخراج RNA انجام شد. برای سنتز cDNA از کیت Superscript RTase استفاده ‌شد و cDNA حاصله با استفاده از پرایمرهای ژن‌های مذکور و ژن بتا اکتین به‌ عنوان ژن رفرنس در Real Time PCR استفاده شد. ارزیابی بیان ژن ویتلوژنین کاهش بیان را در گروه ­های تیمار شده با سم دیازینون نسبت به گروه شاهد نشان داد. در گروه­ های تیمار شده با سم دیازینون (0/8، 1/6 و 3/2 میلی­ گرم بر لیتر) میزان بیان ژن ویتلوژنین به ­ترتیب 0/9، 0/69 و 0/39 برابر گروه شاهد بود که الگوی کاهشی وابسته به دوزی را نشان می دهد (0/05>P). نتایج مطالعه حاضر نشان می ­دهد که سم دیازینون می­ تواند اثر منفی بر رشد و تکامل سلول­ های جنسی در جنس ماده ماهی زبرا داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of sublethal doses of diazinon on on the expression of vitellogenin (Vtg) gene in females Zebrafish (Danio rerio)

نویسندگان [English]

  • Masumeh Darvishi
  • Roughieh Safari
  • Ali Shabany
  • Hosein Hoseinifar
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

The present study investigates, the effects of different doses of diazinon on the expression of vitellogenin (Vtg) gene in females Zebrafish (Danio rerio). For this purpose, 600 Zebrafish with average weight of 0.15+ 0.01 gr in 4 treatments and 3 replicates for one month were exposed to three doses of 0.8, 0.1 and 3.2 mg /ll of diazinon, and a control group for 30 days. At the end of fexpriment,RNA extracted from liver and brain, cDNA syntesised with Superscript RTase kit and PCR were done using primers relate to Vtg and  Beta-actin as housekeeping gene. The evaluation of the expression of the vitellogenin gene showed significant reduction in diazinon-treated groups compared to the control. In the diazinon-treated groups (0.8, 1.6 and 3.2 mg / L), the expression of the vitellogenin gene was 0.9, 0.96 and 0.39 fold of control, respectively, which showed dose-dependent reduction pattern (P≤0.05). The results of this study indicate that diazinone can have a negative effect on the growth and development of sexual cells in zebra fish.

کلیدواژه‌ها [English]

  • Diazinon
  • Vitellogenin
  • Gene expression
  • Zebrafish

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فصلنامه محیط زیست جانوری
دوره 11، شماره 2
مرداد 1398
صفحه 313-318

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