بررسی پاسخ های فیزیولوژیک ماهی سفید (Rutilus frisii) به استرس ناشی از ابزار صید گوشگیر

نوع مقاله: علوم جانوری

نویسندگان

1 گروه شیلات، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

2 پژوهشکده آبزی‌پروری آب های داخلی، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندر انزلی، ایران

3 گروه بیوشیمی، دانشکده علوم، دانشگاه هرمزگان، بندرعباس، ایران

چکیده

برای بسیاری از گونه­ های دریای خزر اطلاعات بسیار اندکی در مورد پاسخ استرس به ابزار و آلات صید و صیادی وجود دارد. در این تحقیق پاسخ­ های فیزیولوژیک ماهیان سفید دریای خزر پس از تنش و استرس حاد وارده توسط ابزار صید گوشگیر مورد ارزیابی قرار گرفت. تور گوشگیر ثابت از ساحل در عمق 0/5 تا 5 متر در دریا مستقر گردید. نمونه­ های خونی برای ارزیابی پاسخ ­های فیزیولوژیک در فواصل زمانی صفر، 1، 3، 6، 12 و 18 ساعت پس از درگیری ماهیان با تور گوشگیر جمع ­آوری گردید. نمونه ­برداری در دو دمای متفاوت (دمای حدود 10 و 15 درجه سانتی­ گراد) انجام گرفت. سطوح کورتیزول، گلوکز پلاسما و پروتئین شوک حرارتی (HSP70) مورد بررسی قرار گرفت. نتایج پارامترهای مذکور نشان داد بین اثر استرس با دمای آب رابطه مستقیم وجود داشت، این نتیجه نشان داد که مقادیر شاخص ­های خونی در دمای بالاتر به نسبت بیش­تر از دمای پایین ­تر بود. مقادیر کورتیزول در هر دو دما اختلاف معنی­ داری بین نمونه ­های خونی در فواصل زمانی را نشان داد (0/05P). سطوح کورتیزول افزایش 100 درصدی را به ­خود اختصاص داده بودند. مقادیر گلوکز پلاسما و پروتئین شوک حرارتی (HSP70) در هر دو دما اختلاف معنی ­داری بین نمونه­ های خونی در فواصل زمانی را نشان نداد (0/05P). در دوره سنجش، مقادیر گلوکز پلاسما و پروتئین شوک حرارتی (HSP70) روند افزایشی داشت که نشان ­دهنده تاثیر زمان استرس بر روی این پارامترها بود. ماهیان درگیر با تور گوشگیر پس از 18 ساعت همگی تلف شدند. شروع این تلفات حدوداً شش ساعت پس از درگیری بود. این مطالعه نشان داد که استرس حاد ناشی از تور گوشگیر بسیار کشنده است و ماهی حتی قادر به تحمل استرس و بقاء را به ­مدت زمان 18 ساعت نیز ندارد.

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