اثر استفاده از سطوح مختلف اکسیدمنیزیم با خلوص بالا بر ویژگی‌های هضمی-تخمیری و تولید متان یک جیرۀ پرکنسانتره در محیط کشت ثابت

نوع مقاله: تغذیه

نویسندگان

1 گروه علوم دامی، مجتمع آموزش عالی تربت جام، تربت جام، ایران

2 گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

 از تغییر الگوی تخمیر در شکمبه، می‌توان در جهت متعادل کردن تولید متان استفاده نمود. اکسید منیزیم علاوه بر تأمین بخش اعظمی از منیزیم مورد نیاز دام، از ظرفیت بافری ویژه‌ای نیز برخوردار است و می‌تواند الگوی تخمیر در شکمبه را دستخوش تغییراتی نماید. در این پژوهش اثر استفاده از یک اکسیدمنیزیم تجاری به ­مقدار 1، 2، 3 و 4 درصد یک جیرۀ پرکنسانتره در یک محیط کشت تهیه شده ازمایع شکمبۀ گوسفند درشرایط in vitro بررسی شد. برخی پارامترهای تخمیری، تجزیه پذیری، تولیدمتان وفراسنجه‌هایتولید گاز ناشی از انکوباسیون جیره در محیط کشت اندازه‌گیری شدند. در اثر افزودن اکسیدمنیزیم، pH محیط کشت، افزایش معنی‌داری نسبت به تیمار شاهد نشان داد ولی اسیدهای چرب فرار کل (TVFA) تغییر نکرد. کم ترین مقدار نیتروژن آمونیاکی (23/26 میلی‌گرم/دسی‌لیتر) در سطح 4 درصد اکسیدمنیزیم مشاهده شد. فراسنجه‌های تولید گاز (گاز 12، 24، 48 و 72 ساعت و نرخ ثابت تولید گاز) و تولید متان در تیمارهای دارای اکسیدمنیزیم،کاهش معنی‌داری نسبت به تیمارشاهد نشان داد. اگرچه ضریب تفکیک پذیری(PF)و تودۀ میکروبی تولیدی، تحت تأثیر تیمارها قرار نگرفت، اما راندمان سنتز تودۀ میکروبی در تیمارهای دارای اکسیدمنیزیم افزایش معنی‌داری نشان داد. به‌نظر می‌رسد که کاربرد اکسیدمنیزیم می‌تواند الگوی تخمیر شکمبه را از طریق کاهش نرخ متان، افزایش قابلیت هضم ماده آلی و راندمان سنتز تودۀ میکروبی، بهبود بخشد، هرچند که انجام آزمایشات بیش ­تر درخصوص تعیین اثرات اکسیدمنیزیم بر روی حیوانات زنده نیز در آینده نیاز است.

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