مقایسه کارایی و ایمنی زایی سه روش تجویز واکسن دوگانه استرپتوکوکوزیس/ لاکتوکوکوزیس در ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss)

نوع مقاله : بیماری ها

نویسندگان

1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 جهاد دانشگاهی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

در این تحقیق کارایی و ایمنی­ زایی تجویز واکسن دو گانه استرپتوکوکوزیس/لاکتوکوکوزیس به سه روش خوراکی، تزریقی و غوطه ­وری در ماهی قزل ­آلای رنگین­ کمان ارزیابی گردید. به این منظور 600 قطعه ماهی قزل­ آلا (2/1±14گرم) به چهار تیمار، هرتیمار در سه تکرار به ­صورت زیر تقسیم گردیدند: تیمار واکسن خوراکی که با خوراک حاوی 108 به­ مدت دو هفته تغذیه گردیدند، تیمار واکسن تزریقی که با 0/1 میلی­ لیتر از باکترین دو باکتری به ­میزان 109 به ­روش داخل صفاقی تزریق گردید و در تیمار غوطه ­وری ماهی­ ها به­ مدت دو دقیقه در سوسپانسیون واکسنی 109 غوطه ­ور گردیدند و تیمار شاهد بدون تجویز واکسن. ماهی­ ها به­ مدت دو ماه تغذیه و نگه ­داری شدند و در روزهای صفر، 20، 40 و 60 شاخص  ­های ایمنی شامل: عیار آنتی ­بادی، فعالیت لایزوزیم، کمپلمان و باکتری­ کشی سرم و نیز میزان پروتئین و گلوبولین سرم بین تیمارها مقایسه گردید. سپس تیمارها با هر دو باکتری به ­طور جداگانه چالش داده شدند و تلفات بین  تیمارها مقایسه گردید. به­ طورکلی می ­توان نتیجه گرفت که علی ­رغم این که تیمار واکسن تزریقی بالاترین میزان کارایی و ایمنی ­زایی را نشان داد، ولی تجویز خوراکی و غوطه ­وری نیز باعث ایجاد محافظت نسبی در ماهی قزل­ آلا گردید و با توجه به ارجحیت ­های روش غوطه ­وری و خوراکی نسبت به تزریقی انجام تحقیق در بهبود کارایی این روش­ ها توصیه می­ گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of efficacy and immunogenicity of three administration routs of Streptococosis/lactococosis vaccine in reinbow trout

نویسندگان [English]

  • Mojtaba Alishahi 1
  • Mostafa Halimi 1
  • Masoud Ghorbanpoor Najaf abadi 2
  • Ahmad Erfanmanesh 3
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahwaz, Iran
3 Jahad Daneshgahi , Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده [English]

In this study  efficacy and immunogenicity of Streptococosis/lactococosis vaccine administrated via oral, bath and i.p routs were investigated. 600 fish (14±2.1g) were randomly divided i 4 similar groups in triplicate as follows: group1 was orally vaccinated with 108 cfu/g food for two weeks. Group2: were injected with 0.1ml of 109  cfu/ml bacterins. Group 3 was vaccinated via immersion rout for 2 minutes in 109  cfu/ml. Group 4: none immunized group without vaccination. Fishes were reared for 2 month in similar situation and blood samples were taken from each group at days zero, 20, 40 and 60 of experiment. Immunological parameters including: antibody titer, serum lysozyme, complement, bactericidal activity and hematological parameters were compared among the groups. Fishes were challenged with S.iniae and L.garviae separately in the end of experiment and rate of mortality compared among the groups. Result showed that rate of mortality in challenged fish with S.iniae in injection, oral, immersion and unvaccinated routs were 20±10,  56.3±10, 50±5.67 and 90± 3.67 whereas in challenged fish with L.garviae were 26.67±5.77, 57.7±10, 43.33±5.67 and 90± 4.63 respectively. Immunological parameters were increased significantly just in injection vaccine treatment increased in sampling periods (P<0.05).  it can be concluded that Although injection rout cause the best protection and immunogenicity, oral and immersion routs induce relative protection without notable immunogenicity in rainbow trout and each rout can be used based on farm situation.

کلیدواژه‌ها [English]

  • Streptococosis/lactococosis vaccine
  • Efficacy
  • [Oral
  • injection
  • immunogenicity
  • rainbow trout
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