هیستومورفولوژی پانکراس و جزایر لانگرهانس در ماهی شبه شوریده بالغ خلیج فارس

نوع مقاله : زیست شناسی (جانوری)

نویسندگان

1 گروه زیست شناسی دریا، دانشکده علوم دریایی، دانشگاه علوم و فنون دریایی خرمشهر

2 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، صندوق پستی: 135

3 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، صندوق پستی: 139

کلیدواژه‌ها


عنوان مقاله [English]

Histomorphology of Pancreas and Langerhans Islets of mature Johnius belangerii in Persian Gulf

نویسندگان [English]

  • Rahim Abdi 1
  • Hassan Morovvati 2
  • Zahra Basir 3
1 Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology
2 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, PO Box: 135
3 Department of basic Science, Faculty of Veterinary Medicine, Ahvaz university of Shahid Chamran
چکیده [English]

In this study, the hepatopancreas of 30 matre Johnius belangerii, were removed and fixed in 10% formalin. The routine procedures of preparation of tissues (specimens, sampling, fixation, washing, dehydration, clearing, impregnation, blocking, sectioning, staining, and mounting) were followed and the paraffin blocks were cut at 6 microns, stained with H&E, PAS and Gomori, then studied under light microscope. In macroscopic observations the hepatopancreas was located in left and extended to the posterior. In microscopic study Pancreas tissue was penetrated into the liver along with portal vein. Pancreas tissue consisted of serous cell clusters as exocrine tissue, which was arranged around a central vein with H&E and PAS staining. The serous cells had a euchromatin nucleus in basal portion, and their basal cytoplasm was basophilic, but the apical cytoplasm contained eosinophilic zymogenic granules. A few pancreatic islets were seen in Gomori staining and only 2 types of cells were recognizable; A or α cells with large and euchromatin nucleus and B or β cells with small and heterochromatin nucleus. Results showed that the hepatopancreas structure of J. belangerii has many similarities to the other fish species.

کلیدواژه‌ها [English]

  • Johnius belangerii
  • histomorphology
  • pancreas
  • Langerhans Islets
  • Persian Gulf