تأثیر افزودن ویتامینC به آب در یک سازگان توأم ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss) و گیاه کاهو (Lactuca sativa) و بررسی عملکردی رشد گیاه و ماهی

نوع مقاله : زیست شناسی (جانوری)


گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران


به­ منظور بررسی اثر افزودن ویتامین C به آب و تاثیر آن بر شاخص ­های رشد و ایمنی در قزل ­آلای رنگین ­کمان (Oncorhynchus mykiss)­ و گیاه کاهو(Lactuca sativa)، در یک سازگان توأم ماهی و گیاه ، تعداد 90 قطعه ماهی قزل­ آلای رنگین ­کمان 1/5±45/00 گرمی و 260 عدد نشا کاهو به­ شکل کاملاً تصادفی به 9 واحد آزمایشی وارد شدند. تیمارها تحت تأثیر­ 0،­ 15 ­ و ­30 میلی ­گرم در لیتر ویتامین C به مدت 60­ روز قرار گرفتند.­ درصد افزایش وزن و نرخ رشد ویژه، نرخ تبدیل غذایی و نرخ کارایی پروتئین در تیمار 30 میلی گرم ویتامین C، اختلاف معنی­ داری را در انتهای دوره آزمایش در مقایسه با سایر تیمار­ها نشان داد (0/05>P). شاخص ­های رشد گیاه کاهو از قبیل طول برگ، عرض برگ، طول بوته، وزن­ تر بوته، وزن خشک بوته، طول ریشه و وزن خشک ریشه در بین تیمار­ها فاقد تفاوت معنی ­دار بود (0/05<P) اما شاخص وزن تر ریشه گیاه کاهو دارای تفاوت معنی ­دار بود (0/05>P). اندازه ­گیری شاخص ­های بیوشیمیایی خون ماهی نیز نشان داد با افزایش سطح 15 میلی­ گرم ویتامین C در هر لیتر، بیش ترین غلظت متوسط هموگلوبین داخل گلبول قرمز (MCHCبه­ مقدار 0/73± 34/13 گرم بر دسی ­لیتر خون ماهی را نشان می ­دهد که این مقدارنسبت به تیمارهای دیگر معنی ­دار بود.­ سایر شاخص ­های خونی از قبیل هماتوکریت (Hct)­، حجم متوسط گلبولی­ (MCV)،­ هموگلوبین­ (HGB)، ­تعداد گلبول­ های قرمز ­(RBC)­ و میانگین هموگلوبین گلبولی­ (MCH) فاقد تفاوت معنی ­دار بودند ­(0/05<P). ­نتایج این آزمایش نشان داد با افزودن 15­ تا ­30 میلی ­گرم بر لیتر ویتامین C به آب، مقدار غلظت متوسط هموگلوبین داخل گلبول قرمز (MCHC)در خون ماهی تغییر می­ کند و رشد ماهی و گیاه نیز بیش ­تر می­ شود.


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