بررسی فعالیت ضدمیکروبی سویه باکتریاییLactococcus lactis subsp. cremoris NABRII66 جداسازی شده از روده ماهی قزل‌آلای رنگین‌کمان

نوع مقاله : بیماری ها

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، ایران

2 مدیریت شمال کشور، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

چکیده

این مطالعه با هدف بررسی فعالیت ضد‌میکروبی­ سویه باکتریایی اسیدلاکتیک Lactococcus lactis subsp. cremoris NABRII66 علیه عوامل باکتریایی بیماریزای شایع در آبزی‌پروری و انسان توسط آزمایش‌های in vitro انجام شد. L. lactis subsp. cremoris NABRII66 از روده ماهی قزل‌آلای رنگین‌کمان جداسازی شده بود. به ­منظور بررسی فعالیت ضدمیکروبی سویه باکتریایی منتخب علیه عوامل بیما‌ریزای باکتریایی تاثیرگذار بر آزادماهیان و سایر گونه‌های ماهیان شامل L.garvieae ، Aeromonas salmonicida و دو عامل بیماریزای دیگر شامل Escherichia coliوStaphylococcus aureus به ­عنوان دو منبع شایع عفونت انسانی، از دو روش‌ آگار دولایه و میکروتیتر پلیت استفاده شد. نتایج  روش آگار دولایه، فعالیت ضد‌میکروبی سویه باکتریایی L. lactis subsp. cremoris NABRII66 را علیه تمامی عوامل بیماریزا نشان داد و بیش ­ترین اثر معنی‌دار بازدارندگی بر مهار رشد باکتری‌های بیماریزای A. salmonicida­ و ­S. aureus مشاهده گردید (0/05>p). هم­ چنین، نتایج تست میکروتیتر پلیت نشان داد که ­سوپرناتانت فاقد سلول سویه باکتریایی L. lactis subsp. cremoris NABRII66 به ­طور معنی‌داری قادر به مهار رشد باکتری A. salmonicida بود (0/05>p). ­این مطالعه نشان داد که سویه باکتریایی L. lactis subsp. cremoris NABRII66 جداسازی شده از روده ماهی قزل‌آلای رنگین­ کمان، دارای پتانسیل ضدمیکروبی علیه چهار عامل باکتریایی بیماریزای شایع توسط تولید برخی ترکیبات برون‌سلولی بوده که باید در مطالعات بعدی مورد ارزیابی قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of the antimicrobial activity of Lactococcus lactis subsp. cremoris NABRII66 bacterial strain isolated from rainbow trout intestine

نویسندگان [English]

  • Faezeh Mortezaei 1
  • Maryam Royan 2
  • Arya Babakhani 1
  • Ramin Seighalani 2
1 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh sara, Iran
2 North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran,
چکیده [English]

This study was conducted to survey the antimicrobial activity of Lactococcus lactis subsp. cremoris NABRII66 lactic acid bacterial strain against common bacterial pathogens in aquaculture and human populations by in vitro experiments. L. lactis subsp. cremoris NABRII66 has been isolated from rainbow trout intestine. Two methods including double layer agar and microtiter plate assay were used to evaluate the inhibition activity of selected bacterial strain against two bacterial pathogens L. garvieae and Aeromonas salmonicida species affecting salmonids and other fish speciesand two other bacterial pathogens including Escherichia coli and Staphylococcus aureus, as two most common source of infection in human population. The results of double layer agar method showed the antimicrobial activity of L. lactis subsp. cremoris NABRII66 bacterial strain against all the tested pathogens and the highest significant growth inhibition zone was observed against A. salmonicida and S. aureus bacterial pathogens (p<0/05). The results of the microtiter plate assay method showed that cell-free supernatant of L. lactis subsp. cremoris NABRII66 bacterial strain could significantly inhibit the growth of A. salmonicida (p<0/05). This study showed that Lactococcus lactis subsp. cremoris NABRII66 bacterial strain isolated from rainbow trout intestine had antimicrobial activity against four aquaculture and human's bacterial pathogens by producing some extracellular inhibitory compounds, which should be evaluated in future studies.

کلیدواژه‌ها [English]

  • aquaculture
  • Lactic acid bacteria
  • bacterial pathogens
  • Antimicrobial activity
  • rainbow trout
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