اثرات سطوح مختلف لایزوزیم جیره غذایی بر فاکتورهای رشد، شاخص های خون شناسی و بیوشیمیایی سرمی در ماهی کپورمعمولی (Cyprinus carpio)

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

گروه شیلات، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

این مطالعه به­ منظور بررسی اثر لایزوزیم در مقیاس­ه ای اضافه شده به جیره در برخی فاکتورهای رشد،  شاخص­ های ایمنی خون شناسی، بیوشیمیایی سرم در ماهی کپور معمولی­ (Cyprinus carpio می­ باشد. به­ این منظور 180 قطعه ­ماهی با میانگین وزنی 0/1±25/94 گرم در 12 عدد تانک و 4 تیمار با 3 تکرار قرار داده شدند. پس از دوره سازگاری، ماهی­ ها با جیره­ های حاوی 0 ، 0/5، 1 و 1/5 گرم بر کیلوگرم لایزوزیم به ­مدت 8 هفته تغذیه شدند. در انتهای دوره ماهیان زیست ­سنجی شدند و خونگیری به ­عمل آمد و شاخص ­های خونی بررسی شد. نتایج حاصل از این مطالعه نشان داد این محرک ایمنی در میـزان رشد به ­میزان حدودأ 26/37 گرم تاثیر مثبت داشته و بین تیمارها اختلاف معنی­ دار مشاهده شد (0/05>p). لایزوزیم جیره بر تعداد گلبول­ های سفید تیمارها با گروه شاهد تفاوت معنی ­داری را نشان دادند. بین تیمارهای آزمایشی در تعداد هموگلوبین نیز روند افزایشی مشاهده شد. شمارش افتراقی گلبول­ های سفید فقط در میزان لنفوسیت­ افزایش معنی­ داری بین تیمارهای آزمایشی و شاهد مشاهده شد، در بررسی میزان نوتروفیل بین تیمارها و شاهد اختلاف معنی ­داری دیده شد.­ هم­ چنین در مطالعه شاخص ­های بیوشیمیایی خون، مشخص شد که در هر سه فاکتور اختلاف معنی­ داری وجود داشت و روند افزایشی داشتند. با توجه به نتایج مطالعه حاضر به­ نظر می­ رسد استفاده از دوزهای ­1­ و ­1/5 گرم بر کیلوگرم لایزوزیم می­ تواند به بهبود فاکتورهای خونی و بیوشیمیایی سرم و رشد این­ گونه پرورشی تاثیر مطلوبی داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of different levels of dietary lysozyme on growth Performance, serum and blood biochemical parameters In common carp (Cyprinus carpio)

نویسندگان [English]

  • Sharare Bakht azad
  • Hamed Paknezhad
  • Seyed Hossein Hosseinifar
  • Abdul Majid Hajimoradloo
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

This study was conducted to evaluate the effects of lysozyme on dietary supplement scales in some growth factors, hematologic immunity indexes, and serum biochemical parameters in common carp (Cyprinus carpio). For this purpose, 180 pieces of fish were weighed 25.94 ± 0.1 g in 12 tanks and 4 treatments with 3 replications. After the adaptation period, fish were fed with diets containing 0, 0.5, 1 and 1.5 g / kg lysozyme for 8 weeks. At the end of the period, the fish were biometric and blood samples were taken and blood indices were examined. The results of this study showed that this immune stimulant had a positive effect on growth rate of about 26/37 grams and there was a significant difference between treatments (p<0.05). Lysozyme diet showed a significant difference between the number of white blood cells and the control group. There was an increasing trend in the number of hemoglobin in the experimental group. The differential count of white blood cells only showed a significant increase in lymphocyte count between treatments and control. There was a significant difference between treatments and control in neutrophil count. Also, in the study of biochemical parameters of blood, we found that there was a significant difference in all three factors and there was an increasing trend. According to the results of this study, it seems that the use of doses of 1 and 1.5 g / kg of lysozyme may have a beneficial effect on the improvement of blood and biochemical parameters of the serum and the growth of this breeding stock.

کلیدواژه‌ها [English]

  • Cyprinus carpio
  • growth performance
  • Hematology
  • Lysozyme enzyme
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