بررسی اثرات افزودن اوره معمولی و اوره آهسته رهش نیتروزا به جیره های حاوی ملاس بر تخمیر میکروبی با روش تولید گاز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی صفی آباد، سازمان تحقیقات، آموزش و ترویج کشاورزی، دزفول، ایران

2 گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران

3 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

این آزمایش با هدف بررسی اثرات نوع و سطح منبع نیتروژن غیرپروتئینی و نیز سطح ملاس افزوده شده به جیره ‏های پُر کنسانتره بر فراسنجه ‏های تولید گاز انجام شد. برای انجام آزمایش از طرح کاملاً تصادفی با الگوی فاکتوریل 3×2×2 با 12 تیمار و 5 تکرار استفاده شد. فاکتورها شامل دو نوع منبع نیتروژن غیرپروتئینی (اوره معمولی و اوره آهسته رهش نیتروزا) و هر منبع در دو سطح (سطوح 0/8 و 1/6 درصد اوره معمولی و سطوح 0/9 و 1/8 درصد اوره آهسته رهش) و ملاس در سه سطح (صفر، 10 و 20 درصد) براساس ماده خشک جیره بودند. با افزایش سطح ملاس مقادیر گاز تولیدی تجمعی تا ساعت 24 انکوباسیون، انرژی متابولیسمی، ماده آلی قابل هضم واقعی، درصد قابلیت هضم ماده آلی و اسیدهای چرب کوتاه زنجیر افزایش یافت (0/05>p ). اثرات نوع منبع نیتروژن غیرپروتئینی و سطح اوره معمولی و آهسته رهش، بر تولید گاز تجمعی، طی مدت انکوباسیون، معنی‏ دار نبود. فراسنجه ‏های تولید گاز تحت تاثیر نوع و سطح منبع نیتروژن غیرپروتئینی (اوره معمولی در مقایسه با اوره آهسته رهش نیتروزا) قرار نگرفت. ماده آلی قابل هضم واقعی در جیره‏ های حاوی اوره آهسته رهش نسبت به اوره معمولی بالاتر بود (0/05>p ) ولی در سایر فراسنجه ‏های اندازه ‏گیری شده تفاوتی بین دو منبع نیتروژن غیرپروتئینی مشاهده نشد. به ­طورکلی نتایج نشان داد که به ­نظر نمی ‏رسد از نظر سرعت آزادسازی آمونیاک در محیط کشت بین اوره معمولی و اوره آهسته رهش نیتروزا تفاوتی وجود داشته باشد، ولی افزودن 20 درصد ملاس سبب بهبود فراسنجه‏ های تولید گاز شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effects of adding conventional urea and slow release urea to diets containing molasses on microbial fermentation by gas production method

نویسندگان [English]

  • Mohammadreza Mashayekhi 1
  • Mohsen Sari 2
  • Naeem Erfani Majd 3
1 Department of Animal Science Research, Safiabad Agricultural Research and Training Center and Natural Resources, Agricultural Research, Education and Extension Organization, Dezful, Iran
2 Department of Animal Sciences, Faculty of Animal Sciences and Food Industry, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

This experiment was conducted to investigate the effects of source and level of non-protein nitrogen, as well as the level of molasses added to high concentrate diets on gas production parameters. A completely randomized design with factorial (2×2×3) arrangement of 12 treatments with 5 replications was used. Factors were two types of non-protein nitrogen sources, common urea and slow-release urea (Nitroza) each in two levels (0.8 and 1.6%) and (0.9 and 1.8%) on dry matter basis of ration respectively, and molasses at three levels (0, 10 and 20%). With increasing of molasses, the amounts of cumulative gas production up to 24 hours of incubation, metabolisable energy, truly organic matter digestibility, organic matter digestibility and short chain fatty acids, increased (p < 0.05). Cumulative gas production was not affected by non-protein nitrogen source and its levels. Truly organic matter digestibility was higher in diets containing slow-release urea than conventional urea, but in other parameters there was no difference between the two non-protein nitrogen sources. In general, the results showed that the rate of ammonia release from conventional and slow release urea in the culture medium does not have a significant effect on in vitro fermentation, but adding 20% molasses on dry matter basis of ration, improved the gas production parameters.

کلیدواژه‌ها [English]

  • Common urea
  • Gas production
  • Molasses
  • Slow Release Urea
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