ارزیابی خصوصیات فیزیکوشیمیایی و فعالیت آنتی اکسیدانی تعدادی از نمونه‌های عسل مناطق جغرافیایی مختلف ایران (فعالیت آنتی اکسیدانی و خواص فیزیکوشیمیایی عسل)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه بهداشت و کنترل کیفی مواد غذایی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

10.22034/aej.2021.139033

چکیده

خصوصیات فیزیکوشیمیایی و آنتی اکسیدان‌های عسل به پوشش گیاهی مورد استفاده زنبور و انواع گیاهان  هر منطقه بستگی دارد. هدف اصلی مطالعه حاضر بررسی خصوصیات فیزیکوشیمیایی و آنتی اکسیدانی عسل مناطق جغرافیایی مختلف ایران و مقایسه بین آن‌ها است. بدین منظور مطالعه ای توصیفی-کاربردی توسط آزمایشات فیزیکوشیمیایی: رطوبت، pH، اسیدیته، میزان خاکستر، قند احیا کننده قبل از هیدرولیز، ساکارز،HMF بر روی 32 نمونه‌ عسل مناطق جغرافیایی مختلف (نواحی جنوب، مرکز، شمال و شمال غربی) ایران انجام شد. مقدار ترکیبات فنولیک با استفاده از معرف Folin-Ciocalteu و فعالیت آنتی اکسیدانی با روش DPPH مورد بررسی قرار گرفت. داده‌های جمع آوری شده با استفاده از نرم افزار آماری SPSS و تست تکمیلی one-way ANOVA تجزیه و تحلیل شدند که سطح معنی داری 0/05 در نظر گرفته می‌شود. مقادیر دامنه به دست آمده از هر آزمون به شرح زیر است: دامنه pH برابر با 4/06 تا 4/39، اسیدیته 8 تا 20 میلی اکی والان در کیلوگرم، رطوبت 14/9 تا 18/8 درصد، خاکستر 0/2 تا 0/6 درصد، میزان HMF 4/49 تا 17/55 میلی‌گرم در کیلوگرم، DPPH 32/37 تا 74/77 درصد و محتوای فنولیک 12/69 تا 54/66 میلی‌گرم در 100 گرم بود (0/05>P). دامنه قند احیاکننده 71/42 تا 80/88 درصد، ساکارز 1/7 تا 4/88 درصد بود (05/0>P). از نظر pH، اسیدیته، رطوبت، خاکستر، میزان هیدروکسی متیل فورفورال، DPPH و میزان فنل بین مناطق مختلف تولید عسل تفاوت معنی داری وجود داشت. هیچ تفاوت معنی داری بین محتوای ساکارز و قند قبل از هیدرولیز نمونه‌های عسل‌های مناطق مختلف وجود ندارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Physicochemical Properties and Antioxidant Activity of Honey in Different Geographical Regions of Iran

نویسندگان [English]

  • Abolfazl Kamkar
  • Fatemeh Gheitanchi
  • Hessameddin Akbarein
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده [English]

Honey is a natural sweet substance which the physico-chemical properties and antioxidants of honey are depended to the kinds of plant and flower in each region. Therefore, it is required to know components of each. The main aim of current study was to investigate the physiochemical and antioxidant properties of 32 different samples from different regions (South, central, North and Northwestern) of Iran. Thus, physiochemical tests such as moisture content, pH, acidity, Ash content, reducing sugar before hydrolysis, sucrose, HMF on honey samples were done. Also the total amount of phenolic compounds using Folin-Ciocalteu reagent and antioxidant activity was evaluated by DPPH method. The collected data were coded and analyzed through SPSS by applying one-way ANOVA.The results depicted significant (P<0.05). The obtained range values of each test is as follows: The range of pH was 4.06-4.39, acidity 8-20 mEq/kg, moisture 14.9-18.8%, ash 0.2-0.6%, HMF 4.49-17.55 mg/kg, reduced sugar 71.42-80.88%, sucrose 1.7-4.88%, DPPH 32.37-74.77% and phenolic content was 12.69-54.66 mg/100g. We found significant differences (P < 0.05) in pH, acidity, moisture, ash, HMF, DPPH and phenolic content between different regions of producing honey but not with sucrose and reduced sugar. Our results are consistent with previous findings and CODEX standards.

کلیدواژه‌ها [English]

  • honey
  • Physicochemical properties
  • Antioxidant activity
  • phenolic content
  • geographical regions
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