تاثیر افزایش مواد معدنی در جیره غذایی بر شاخص های رشد، فلور باکتریایی و مورفولوژی روده ماهی تیلاپیای نیل (Oreochromis niloticus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه شیلات، دانشکده مهندسی منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران

10.22034/aej.2021.139159

چکیده

هدف از این مطالعه، بررسی اثر افزایش مواد معدنی جیره بر شاخص های رشد و تغییر فلور باکتریایی روده و سلامتی ماهی تیلاپیای (Oreochromis sp) در یک سازگان مدار بسته بود. به این منظور 180 قطعه ماهی تیلاپیا  با متوسط وزن 3/8±30 گرم به 9 واحد آزمایشی معرفی گردید (3 واحد آزمایشی برای هر تیمار). تیمارهای آزمایش شامل سطوح مختلف 0، 25 و 75 درصد غلظت مواد مغذی در جیره غذایی با توجه به براورد نیاز گیاه در یک دوره پرورش در سیستم هیدروپونیک معمولی طراحی گردید. پس از طی 70 روز، از ساقه دمی 5 ماهی از هر تیمار خونگیری به عمل آمد و بافت روده جهت بررسی فلور باکتریایی و بافت شناسی نیز نمونه ­برداری گردید. نتایج به دست آمده حاکی از تغییر معنی­ دار (0/05>P) تعداد کل باکتری­ های روده تنها در تیمار حاوی 75 درصد مواد معدنی در مقایسه با سایر تیمارها بود (0/05>P). نتایج نشان داد که جیره­ های حاوی مواد معدنی  تاثیر معنی ­داری بر شاخص ­های بافت ­شناسی روده نظیر طول و قطر پرز، تعداد سلول­ های لوکوسیت و تعداد سلول­ های جامی دارد. به طور کلی، رشد و توسعه اپیتلیوم روده، احتمالاً با فزایش مواد معدنی در جیره تیلاپیا، شرایط زیستی بهتری را برای این ماهی ایجاد می کند.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of different levels of inorganic supplement in hydroponic system on hemato-immunological responses, intestine bacterial flora and morphology of Nile tilapia (Oreochromis niloticus)

نویسندگان [English]

  • Ebrahim Motaghi 1
  • Gholamreza Rafiee 1
  • Alireza Mirvaghefi 1
  • Eisa Ebrahimi 2
  • Bagher Majazi Amiri 1
1 Department of Fisheries, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Department of Fisheries, Faculty of Natural Resources Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

The aim of the present study is to investigate the effect of different levels of inorganic supplements in hydroponic system with the re-use of water on some parameters related to Nile tilapia health status. To address that, 180 fish weighing 30±3.8 g was divided into 9 experimental units (3 for each treatment). Three different treatments were applied, including 0, 25 and 75% of nutrient requirement for hydroponic system and these levels were added to the diet of fish. Following 70 days of dietary administration, blood samples were withdrawn for caudal vein of 5 fish. Intestine sample was also taken in order to determine the bacterial microflora and histological changes. Obtained results showed significant changed in hematological and immunological parameters in dietary treatment as compared with control (P<0.05). Total number of bacteria in intestine was significantly (P<0.05) elevated in treatment 3 as compared with other treatments. The applied diets had significant impacts on histological indices, including villi height and diameter, total number of leucocyte and mucus-secreting cells (P<0.05). Generally, epithelium development of intestine besides the improvement of hematological parameters and stimulation of immunity system could perhaps indicate the better health status of Nile tilapia following dietary manipulation by inorganic supplementation.

کلیدواژه‌ها [English]

  • Intestinal microflora
  • Lysozyme activity
  • Hemoglobin
  • Mucus-Secreting cells
  • Aquaponic
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