بررسی میزان شیوع مولکولی توکسوپلاسما گوندی در نمونه های شیر دامی جمع آوری شده از استان البرز به روش PCR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه بهداشت مواد غذایی، دانشکده دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

2 مرکز تحقیقات مراقبت های بالینی و ارتقا سلامت، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

3 گروه انگل شناسی، دانشکده دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

10.22034/AEJ.2021.297509.2593

چکیده

مصرف مواد غذایی خوب حرارت داده نشده با منشا دامی یکی از اصلی ترین دلایل ابتلا به بیماری توکسوپلاسموزیس است. پژوهش کنونی به منظور ارزیابی میزان شیوع توکسوپلاسما گوندی در نمونه های شیر خام جمع آوری شده از استان البرز انجام شد. 350 نمونه شیر خام گاو، گوسفند و بز جمع آوری و به آزمایشگاه انتقال داده شد. استخراج DNA  از نمونه ها انجام و ردیابی ژن B1 توکسوپلاسما گوندی با استفاده از واکنش زنجیره ای پلی مراز مورد استفاده قرار گرفت. 30 نمونه از کل 350 نمونه شیر خام ارزیابی شده، حاوی ژن B1 توکسوپلاسما گوندی بودند (8/57 درصد). شیوع مولکولی توکسوپلاسما گوندی در نمونه های شیر خام گاو، گوسفند و بز به ترتیب( 5/33 ، 12/00 و 10) درصد بود. شیر خام و پاستوریزه نشده گوسفند به عنوان بیش ترین منبع خطر برای انتقال توکسوپلاسما گوندی در نظر گرفته می شود.  جوشش کامل شیر خام و استفاده از شیر پاستوریزه برای کاهش خطر ابتلا به توکسوپلاسما گوندی توصیه می شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study the molecular prevalence rate of Toxoplasma gondii in raw milk samples collected from Alborz province by PCR method

نویسندگان [English]

  • Mohammadreza Meftahi 1
  • Zohreh Mashak 2
  • Marzieh kefayat 3
1 Department of Food Hygiene, Faculty of Veterinary, Karaj Branch, Islamic Azad University, Karaj, Iran
2 Clinical Care and Health Promotion Research Center, Karaj branch, Islamic Azad University, Karaj, Iran
3 Department of parasitology, Faculty of Veterinary, Karaj Branch, Islamic Azad University, Karaj, Iran
چکیده [English]

Consumption of good unheated food of animal origin is one of the main causes of Toxoplasmosis. The present study was performed to evaluate the prevalence of Toxoplasma gondii in raw milk samples collected from Alborz province. 350 samples of raw milk of cows, sheep and goats were collected and transferred to the laboratory. DNA extraction was performed from the samples and Toxoplasma gondii B1 gene was detected using a polymerase chain reaction. 30 samples out of 350 samples of raw milk evaluated contained Toxoplasma gondii B1 gene (8.57%). The molecular prevalence of Toxoplasma gondii in raw milk samples of cattle, sheep and goats was (5.33, 12.00 and 10) percent, respectively. Raw and unpasteurized sheep milk is considered to be the highest risk source for Toxoplasma gondii transmission. Boiling raw milk and using pasteurized milk is recommended to reduce the risk of Toxoplasma gondii.

کلیدواژه‌ها [English]

  • Toxoplasma gondii
  • Raw milk
  • Molecular prevalence
  • Alborz province
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