بررسی اثر سطوح مختلف اسید فولیک بر عملکرد رشد، ترکیب لاشه و شاخص های خونی بچه فیل ماهیان انگشت قد (Huso huso Linnaeus 1758)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

2 پژوهشکده آبزی پروری آب های داخلی، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرانزلی، ایران

3 گروه تخصصی شیلات و آبزیان، موسسه آموزش و ترویج کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

10.22034/AEJ.2021.295613.2586

چکیده

اسید فولیک نقش اساسی در ارتقاء ایمنی آبزیان ایفا می‏ کند. به منظور بررسی اثر رژیم غذایی اسید فولیک بر عملکرد رشد، ترکیبات لاشه، پارامترهای خونی از 270 عدد بچه فیل ماهی (Huso huso) انگشت قد با میانگین وزنی 8-5 گرم با سطوح مختلف اسید فولیک شامل: 0/6 میلی ­گرم در کیلوگرم (تیمار شاهد 1)، 2/68 میلی ­گرم در کیلوگرم (تیمار 2)، 3/72 میلی ­گرم در کیلوگرم (تیمار3) و 4/84 میلی ­گرم در کیلوگرم (تیمار4)، با سه تکرار به مدت 56 روز استفاده شد. اثر تیمارهای آزمایشی بر عملکرد رشد و شاخص های ایمنی بررسی گردید. نتایج نشان داد؛ اثر تیمارهای آزمایشی بر ضریب تبدیل خوراک، نرخ رشد ویژه، بازده خوراک و افزایش وزن معنی ­داری بود (0/05>P). سطوح مختلف اسیدفولیک بر تعداد گلبول های سفید نیز اختلاف معنی داری ایجاد نمود (0/05>P)؛ به طوری که کم ترین مقدار آن در تیمار شاهد به میزان 3 10×mm-3  62/4 و بیش ترین آن به میزان  103×mm-3  75/6 در تیمار چهارم مشاهده شد. تعداد لنفوسیت ها در بین گروه های آزمایشی اختلاف معنی دار داشت (0/05>P) ولی اختلاف معنی داری در تعداد نوتروفیل ها دیده نشد (0/05<P). پس از آنالیز لاشه، همان طور که انتظار می رفت افزایش سطح اسیدفولیک جیره سبب افزایش معنی دار درصد پروتیین خام در لاشه گردید (0/05>P). به طوری که بیش ترین مقدار درصد پروتیین خام در تیمار سوم به میزان 2/13 مشهود بود. شاخص های رطوب، چربی و خاکستر لاشه به طور معنی داری تحت تاثیر تیمارهای ازمایشی قرار نگرفتند. یافته‌های این آزمایش نشان داد که با افزایش میزان اسیدفولیک به میزان 3/72 میلی گرم در کیلوگرم درجیره، ماهی ها مقاومت بهتری از نظر ایمنی و عملکرد رشد از خود نشان خواهند داد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of different levels of folic acid on growth performance and blood indexes of Beluga fingerling (Huso huso Linnaeus 1758)

نویسندگان [English]

  • Ataollah Yeganehkari 1
  • Hadi Ershad Langroudi 1
  • Alireza Valipour 2
  • Soheil Alinejad 3
1 Department of fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agriculture Research Education and Organization (AREEO), Bandar Anzali, Iran
3 Department of Fisheries and Aquatic, Institute of Agricultural Education and Extension, Agriculture Research Education and Organization (AREEO), Tehran, Iran
چکیده [English]

Folic acid plays an essential role in promoting aquatic immune. In order to investigate the effect of folic acid diet on growth performance, carcass composition, blood parameters of 270 beluga fingerling with an average weight of 5-8 g with different levels of folic acid, including: 0.6 (as a control), 2.68, 3.72 and 4.84 mg/kg with three replications for 56 days were considered. The effect of experimental treatments on growth performance and immune indices was investigated. Results showed that the effect of treatments on feed conversion, specific growth rate, feed efficiency and weight gain was significant (P<0.05). Different levels of folic acid also made a significant difference in the number of white blood cells (P<0.05); So that the lowest value in the control treatment was 4.62 mg/kg and the highest was 6.75 mg/kg in the fourth treatment. There was a significant difference in the number of lymphocytes between the experimental groups, but no significant difference was observed in the number of neutrophils. After carcass analysis, as expected, increasing dietary folic acid level caused a significant increase in the percentage of crude protein in the carcass. So that the highest percentage of crude protein in the third treatment was 2.13 mg/kg. Moisture, fat and ash carcass indices were not significantly affected by experimental treatments. The results of this experiment showed that by increasing the amount of folic acid by 3.72 mg/kg, the fish will show better resistance in terms of immune and growth performance.

کلیدواژه‌ها [English]

  • Folic acid
  • Blood serum
  • Growth function
  • Immunity index
  • Beluga
  • Huso huso
  1. Kenari, A.A., Regenstein, J.M., Hosseini, S.V., Rezaei, M., Tahergorabi, R., Nazari, R.M. and Kaboli, S.A., 2009. Amino acid and fatty acid composition of cultured Beluga (Huso huso) of different ages. Journal of Aquatic Food Product Technology. 18(3): 245-265. ‏
  2. Mohseni, M., Ozorio, R., Pourkazemi, M. and Bai, S., 2008. Effect of dietary L-Carnitine supplements on growth and body composition in Beluga sturgeon (Huso huso) juveniles. Journal of applied ichthyology. 24(6): 646-649.
  3. Mohammadnejad Shamushki, M. and Mazini, M., 2012. The effect of probiotic baker's yeast (Saccharomyces cerevisiae) on the growth and survival of sea bream (Rutilus rutilus caspicus). Journal of Aquaculture Development. 6(1): 103-111. (In Persian)
  4. OlivaTeles, A., 2012. Nutrition and health of aquaculture fish. Journal of fish diseases. 35(2): 83-108.
  5. Shahkar, A., Khara, H. and Sudagar, M., 2017. The effect of feeding frequency on the growth rate and survival rate of Rutilus frisii kutum Journal of Biological Sciences. Lahijan branch. 2(3): 41-49. (In Persian)
  6. GalkandaArachchige, H.S., Wilson, A.E. and Davis, D.A., 2020. Success of fishmeal replacement through poultry by‐product meal in aquaculture feed formulations: a meta‐Reviews in Aquaculture. 12(3): 1624-1636. ‏
  7. Safari, R., Khani, F. and Haghipoor, , 2016. Comparison of some plasma and serum Biochemical parameters in Great sturgeon (Huso huso), Persian sturgeon (Acipenser persicus) and Caspian roach (Rutilus rutilus caspicus). Journal of Animal Environmental. 7(4): 193-200. (In Persian)
  8. Jedi Mostafalou, A., Hedayati Fard, M., Keshavarz Divkolaie, M. and Mohamadyan, T., 2021. Comparative Study of Different Levels of Sodium Di-formate (NDF) and Formic Acidifiers on Nutrition, Growth and Activity of Digestive Enzymes in beluga (Huso huso). Journal of Animal Environmental. 13(1): 247-258. (In Persian)
  9. Lin, Y.H., Lin, H.Y. and Shiau, S.Y., 2011. Dietary folic acid requirment of grouper, Epinephelus malabaricus, and its effects on non-specific immune responses. Aquaculture. 317: 133-137.
  10. Jamalzad Falah, F., Rajabi Islami, H. and Shamsaie Mehrgan, M., 2020. Dietary folic acid improved growth performance, immuno-physiological response and antioxidant status of fingerling Siberian sturgeon, Acipenser baerii(Brandt 1896). Aquaculture Rep. 17:100391. 10.1016/j.aqrep.2020.100391. 
  11. Delsoz Khaki, N., Khara, H., Mohseni, and Shenavar Masoleh, A., 2013. Effects of Bactocell Probiotic (Pediococcus acidilactici) and Folic Acidon Immune System on Ship Sturgeon Fingerling. Journal of Animal Physiology and Development. 6(4): 1-13. (In Persian)
  12. Naderi, M., Khara, H. and Yazdani Sadati, M., 2016. Effects Dietary Vitamin C and Folic Acid on Growth Performance, Hematological and Immunological Parameters of Juvenile Barbel Sturgeon Acipenser nudiventris. Journal of Animal Physiology and Development. 10(1): 67-77. (In Persian)
  13. Falahatkar, B., Akhavan, S.R., Poursaeid, S. and Hasirbaf, E., 2014. Use of sex steroid profiles and hematological indices to identify perinucleolus and migratory gonadal stages of captive Siberian sturgeon Acipenser baerii (Brandt, 1869) females. Journal of applied Ichthyology. 30(6): 1578-1584.
  14. Merrifield, D.L., Dimitroglou, A., Foey, A., Davies, S.J., Baker, R.T., Bøgwald, J. and Ringø, E., The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture. 302(1): 1-18.
  15.  AOAC. 2000. Official methods of analysis of official Analytical chemists.
  16. Rad, A.E., Alishahi, M., Ghorbanpour, M. and Zarei, M., The effects of oral administration of extracted chitosan from white leg shrimp (Litopenaeus vannamei) on hematological and growth indices in common carp (Cyprinus carpio). Journal of Veterinary Research. 69: 4.
  17. Kazemi, R., Pour Dehghani, M., Yousefi Jourdehi, A., Yarmohammadi, M. and Nasri Tejan, M., 2018. Physiology of the circulatory system of aquatic animals and applied techniques of fish hematology. Bazargan Publications. 194 p. (In Persian)
  18. Falahatkar, b., 2013. Aquatic nutrition and ration writing. Publications of the Institute of Applied Scientific Higher Education, Jihad Agriculture, Tehran. 334 p. (In Persian)
  19. Bronzi, P., Rosenthal, H., Arati, G. and Williot, P., 1999. A brief overview on the status and prospects of sturgeon farming in western and central Europe. J. Appl. Ichthyol. 15: 224-227.
  20. Yasemi, M., Poursaeid, S., Shakoorian, M. and Falahatkar, B., 2011. Rearing of great sturgeon, Huso huso, at early stage of growth in earthen ponds. Journal of Applied Ichthyology. 27(2): 576-580.‏
  21. Sesay, D.F., Tsion, H.M., Zhou, Q., Ren, M., Xie, J., Liu, B., Chen, C. and Pan, L., 2016. Effects of dietary folic acid on the growth, digestive enzyme activity, and immune response and antioxidant enzyme activity of blunt snout bream (Megalobrama amblycephala) fingerling. Aquaculture. 452: 142-150.
  22. NRC. 2011. National Research Council. Nutrient requirements of fish and shrimp. Washington, DC.
  23. Barros, M.M., Ranzani-Paiva, M.J.T., Pezzato, L.E., Falcon, D.R. and Guimaraes, I.G., 2009. Haematological response and growth performance of Nile Tilapia (Oreochromis Niloticus) fed diet containing Folic Acid. Aquaculture research. 40(8): 859-903.
  24. Seunghyung, L., Ozan, S., Silas, S.O., James, H. and Fadel, G., 2017. Development of growth rate, body lipid, moisture, and energy models for white sturgeon (Acipenser transmontanus) fed at various feeding rates. Animal Nutrition Market. 3(1): 46-60.
  25. Yan, L., Jinping, W., Xihua, Ch., Zhipeng, Ch. and Jiali, J., 2020. Dietary myo‐inositol requirements of juvenile hybrid sturgeon (Acipenser baerii ♀ × A. schrenckii ♂). Aquaculture research. 51(3): 1143-1150.
  26. Cowey, C.B. and Woodward, B., 1993. The dietary requirement of young rainbow trout (Oncorhynchus mykiss) for folic acid. Journal of nutrition. 123(9): 1594-1600.
  27. Shiau, S.Y. and Huang, S.Y., 2011. Dietary folic acid requirement for maximal growth of juvenile tilapia, Oreochromis niloticus× aureus. Fisheries Science. 67: 655-659.
  28. Esmaeili, B. and Khara, H., 2014. Growth performance, hematology and immunological parameters of rainbow trout, Oncorhynchus mykiss, fed with diets containing different levels of vitamin E and folic acid. Iranian Journal of Fisheries Sciences. 13(4): 931-943.
  29. Maynard, C., Cummins, I., Green, J. and Weinkove, D., 2018. A bacterial route for folic acidsupplementation. BMC Biology. 16(1): 1-10.
  30. Naderi, M., Nezami, Sh., Yazdani sadati, M.A., and Khara, H., 2013. Interaction effect of Vitamin C and Folic Acid on growth factor in Ship (Acipenser nudiventris) Fingerlings, Aquatic animals &Fisheries 4(15): 54-57. (In Persian)
  31. Scully, C., 2014. 8 - haematology. In: Scully, C. (Ed.), Scully’s Medical Problems in Dentistry, seventh edition. Churchill Livingstone, Oxford. 212-275.