تاثیر نانوذرات نقره بر ترکیب لاشه ماهی قرمز (Carassius auratus) تغذیه شده با الیگوساکارید رافینوز و باکتری Pediococcus acidilactici

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

1 گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 مرکز تحقیقات آب های دور، موسسه تحقیقات شیلات ایران، سازمان تحقیقات، ترویج و آموزش کشاورزی، چابهار، ایران

چکیده

هدف از تحقیق حاضر بررسی تاثیر حفاظتی پیش تیمار باکتری Pediococcus acidilactic و الیگوساکارید رافینوز بر ترکیب لاشه در ماهی قرمز (carassius auratus) در مواجهه با نانو نقره بود. بر این اساس تعداد 250 عدد ماهی قرمز با میانگین وزنی 0/18±26/3 در چهار تیمار و هر تیمار با سه تکرار شامل: غذای بدون مکمل غذایی (تیمار1شاهد)، غذای حاوی پروبیوتیک باکتری با غلظت 107 کلنی بر گرم غذا (تیمار2)، غذای حاوی رافینوز به ­میزان 1 گرم در کیلوگرم غذا (تیمار3) و غذای سین بیوتیک (تیمار 4) تقسیم شد و به ­مدت 6 هفته تغذیه شدند. بعد از پایان دوره تغذیه، به تیمارهای آزمایش میزان 50 درصد غلظت کشنده نانو نقره (0/5 میلی­ گرم بر لیتر) به ­مدت 14 روز اضافه ‌شد و در پایان دوره 14 روزه، نمونه ­برداری از لاشه صورت گرفت. تجزیه و تحلیل داده­ ها در پایان دوره آزمایش نشان داد شاخص ­های رطوبت، خاکستر، پروتئین و چربی لاشه در بین تیمارهای آزمایش اختلاف معنی­ داری با هم  نداشتند (0/05<P). هرچند در برخی از شاخص ­ها ازجمله رطوبت، پروتئین و خاکستر در تیمار سین بیوتیک روند افزایشی را نشان داد ولی این افزایش معنی­ دار نبود (0/05<P). با توجه به نتایج به ­دست آمده از این پژوهش و عدم معنی ­داری شاخص­ های مربوطه در تیمارهای مذکور به ­نظر می­ رسد استفاده از این مکمل­ ها با این غلظت اثرگذاری مناسب را بر شاخص ­های مربوطه ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of silver nano particles on carcass composition of goldfish (Carassius auratus) pre-treated with Raffinose oligosaccharide and Pediococcus acidilactici bacteria

نویسندگان [English]

  • Fateme zahra Jafari 1
  • Seyeyd Aliakbar Hedayati 1
  • Seyed Hossein Hoseinifar 1
  • Ali Jafar nodeh 1
  • Tahere Bagheri 2
1 Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Far Water Research Center, Fisheries Research Institute of Iran, Agricultural Research, Extension and Training Organization, Chabahar, Iran
چکیده [English]

Aim of this study was to investigate the protective effect of pre-treatment of bacteria pediococcus acidilactici and oligosaccharideRaffinose on carcass composition of Goldfish (carassius auratus) in exposure to nano silver. Accordingly, 250 fish with an average weighing of 26.3±0.18 fed for 6 weeks in four treatments and each treatment with three replications including diet without food supplement (control 1), probiotic diet of bacteria with a concentration of 107 colonies per gram (treatment 2), food containing probiotic Raffinose (1 g/kg) and synbiotic (treatment 4). After the end of the feeding period, 50% of the nano-silver concentration (0.5 mg/L) was exposed to the treatments for 14 days and at the end of the 14-day exposure, carcass sampling was performed. At the end of the experiment, analysis of data showed that there was no significant difference in the moisture content, ash, protein and carcass fat among the treatments (P>0.05). However, in some indices, such as moisture, protein and ash, there were increased trend in synbiotic treatment, but this increase was not significant (P>0.05). Regarding the results of this study and the lack of meaning of relevant indices in these treatments, it seems that using studied supplements with this concentration does not had an appropriate effect on the studied indices.

کلیدواژه‌ها [English]

  • Probiotic
  • Prebiotic
  • Nano-silver
  • Hematology
  • goldfish
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