Investigation of Amino Acids composition in Common Carp (Cyprinus carpio Linnaeus, 1758) using High-performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC) from the southern of Caspian Sea

Document Type : Biology(Animal)

Authors

1 Department of Fisheries, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Biology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

3 Department of chemistry, Faculty of Chemistry, Shahid Beheshti University, Tehran, Iran

Abstract

The present study aimed to isolate and detect essential amino acids (EAAs) in muscle tissue of common carp (Cyprinus carpio). Fish contains the highest protein content. Three pieces of common carp were collected from gillnets found on beaches of the southern Caspian Sea. Biometrics of collected samples were determined. Target tissue (muscle) of the fish was isolated and kept in nitrogen tanks at -196°C. Dissolution/deposition techniques were used to extract protein from the fish flesh at the isoelectric point, proteins were hydrolyzed into amino acids, and thin layer chromatography (TLC) was used to isolate essential amino acids in the laboratory. Commercial TLC silica gel 60 F₂₅₄ plates (made by Merck, Germany) were used as the stationary phase and butane/acetic acid/water as the mobile phase. Ninhydrin reagent was used to detect amino acids. Quantitative amino acid analysis was carried out and the retention factor (Rf) was calculated. TLC results showed that all EEAs (Arginine, Histidine, Phenylalanine, Isoleucine, Threonine, Valine, Leucine, Methionine and Lysine) were present in muscle tissue of common carp. Tryptophan was totally destroyed with acid hydrolysis and not detected in the analysis. Analysis of 17 essential and nonessential amino acids showed that the highest EAA content of fish flesh belonged to Lysine, Glutamic acid and the lowest content belong to Aspartic acid and Methionine. Since human body cannot synthesize EAAs, common carp should be included in human diet because it is a general source of nutritional components that contribute to growth, energy production, muscle building and treatment of many diseases.

Keywords

References

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Journal of Animal Environment
Volume 12, Issue 3
June 2020
Pages 207-214

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