اثرات واکسن ضد استرپتوکوکوزیس به روش حمام بر سطوح کورتیزول و لاکتات دهیدروژناز LDH)) و بیان ژن های وابسته به ایمنی در ماهی سی باس آسیایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، پیشوا، ایران

2 گروه شیلات و زیست شناسی دریا، پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

3 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، تهران، ایران

4 دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

5 گروه زیست شناسی، دانشکده علوم پایه، واحد واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

10.22034/aej.2024.453286.3123

چکیده

هدف از این مطالعه بررسی اثرات واکسن غوطه‌وری علیه بیماری‌های استرپتوکوکوزیس و لاکتوکوکوزیس (گاروواک) در وزن‌های مختلف بر شاخص های رشد، تغذیه و میزان بازماندگی ماهی سی باس آسیایی (Lates calcarifer) بود. ماهیان با میانگین وزنی 5 گرم در مخازن مدور 300 لیتری پلی اتیلنی در 2 تیمار و 3 تکرار شامل تیمارهای واکسینه شده در وزن 5 گرم (ST) و گروه شاهد بدون اعمال واکسیناسیون مورد بررسی قرار گرفتند. غذادهی دو بار در روز و تا حد سیری به مدت 8 هفته انجام شد. نتایج مربوط به چالش باکتریایی نشان داد که میزان تلفات در تیمار واکسینه شده به صورت معنی‌داری نسبت به گروه شاهد کم‌تر بود (0/05>P). با توجه به نتایج کلی این مطالعه، تجویز واکسن غوطه‌وری گاروواک توانسته است در ماهی سی باس آسیایی با وزن 5 گرم میزان بازماندگی بعد از چالش باکتریایی را بهبود ببخشد. در تحلیل آماری نتایج به‌دست آماده پژوهش حاضر با استفاده از آزمون‌های نرم‌افزار SPSS، بیان ژن‌های مرتبط با ایمنی با استفاده از Real-time (RT)-PCR که تفاوت معنی‌داری در بیان ژن نمونه‌های تیمار با نمونه‌های شاهد مشاهده شد. هم‌چنین میانگین سطوح آنزیم‌های کورتیزول و لاکتات دهیدروژناز در تیمارهای مورد آزمایش ng/g 107و u/kg 1279 که اختلاف معنی‌داری با گروه شاهد نداشت. هم چنین در بررسی آماری بین داده‌های بیان ژنی (Lysozymes،lL-IB،TNF،MHCI، MHCII و IGF-I) اختلاف‌معنی‌داری مشاهده نشد. هرچند میانگین بیان ژنی(Lysozymes،lL-IB،TNF،MHCI، MHCII و IGF-I) در تیمار واکسینه شده بالاتر از میانگین مقادیر تیمار شاهد بود. نتایج به‌دست آمده در پژوهش کنونی نشان داد واکسن، تأثیر بهتری بر سلامت سی‌باس آسیای در وزن 5 گرم داشته است. ولی بر روی سطوح کورتیزول و لاکتات دهیدروژناز اثر معنی داری نداشت. در حالی که بر روی بیان ژنی MHC1 و اینترلوکین 1B تاثیر معنی‌داری داشت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effects of anti-Streptococcosis vaccine by bath method on the levels of cortisol and lactate dehydrogenase (LDH) and the expression of immune-related genes in Asian seabass (Lates calcarifer)

نویسندگان [English]

  • Siamak Yousefi Siahkalroodi 1
  • Ahmad Ghasemi 2
  • Parastoo Mohebi derakhsh 3
  • Mahyar Yousefi siahkalroodi 4
  • Taregh Jamil Neameh 5
1 Department of Biology, Faculty of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran
2 Department of Fisheries, Persian Gulf Research Institute, Persian Gulf University, Bushehr, Iran
3 Iranian Fisheries Science Research Institute, Agriculture Research Education and Organization , Tehran, Iran
4 Faculty of Veterinary Medicine, Science and Research Unit, Islamic Azad University, Tehran, Iran
5 Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

The aim of this study was to investigate the effects of immersion vaccine (Garovak) against streptococcosis and lactococcosis in different weights on the growth, feeding and survival rates of Asian seabass (Lates calcarifer). Fish with an average weight of 5 gr were studied in 300-liter round polyethylene tanks in 2 treatments and 3 repetitions, including the vaccinated treatments at a weight of 5 grams (ST) and the control group without vaccination. Feeding was done twice a day until satiety for 8 weeks. The results related to the bacterial challenge showed that the death rate in the vaccinated treatment was significantly lower than the control group (P<0.05). According to the general results of this study, the administration of Garovak immersion vaccine has been able to improve the survival rate after bacterial challenge in Asian seabass weighing 5 grams. In the statistical analysis of the results of the present study using SPSS software tests, the expression of genes related to immunity using Real-time (RT)-PCR, which has a significant difference in the gene expression of treatment samples was observed with control samples. Also, the average levels of cortisol and lactate dehydrogenase (LDH) enzymes in the tested treatments were 107 ng/g and 1279 u/kg, which had no significant difference with the control group. Also, in the statistical analysis, no significant difference was observed between gene expression data (Lysozymes, TNF, MHCI, and IGF-I). But in 1L-IB and MHCII, was seen significant difference between treatments and controls. However, the average gene expression (Lysozymes, 1L-IB, TNF, MHCI, MHCII and IGF-I) in the vaccinated treatment was higher than the average values of the control treatment. The results obtained in the current study showed that the vaccine had a better effect on the health of Asian sea bass weighing 5 gr. According to the general results of this study, the vaccine has been able to have a positive effect on fish health indicators, but on cortisol and lactate dehydrogenase enzymes (LDH) and also gene expression (Lysozymes, TNF, MHCI, and IGF-I) had no significant effect. The results obtained in the current study showed that the vaccine had a better effect on the health of Asian sea bass weighing 5 grams. But it had no significant effect on the levels of cortisol and lactate dehydrogenase (LDH). While it had a significant effect on MHC1 and interleukin 1B gene expression.

کلیدواژه‌ها [English]

  • Vaccine
  • Streptococcosis
  • Asian seabass
  • Cortisol
  • Lactate dehydrogenase (LDH)
  • Gene expression
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