1
Department of Fisheries Faculty of Natural Resources College of Agriculture & Natural Resources,University of Tehran, Karaj
2
Department of Biochemistry, School of Veterinary medicine, University of Tehran
Abstract
Nowadays, organophosphorus pesticides such as diazinon are used in various agricultural activities and controlling insects' and pests' control. They could enter surface even underground water sources during drainage process. Diazinon can cause reactive oxidative stresses (ROS) during metabolism process in the body of aquatic organisms. The aim of this study is to investigate the antioxidant effect of vitamin C on antioxidant enzyme activities and lipid peroxidation in Oncorhynchus mykiss. Sixty Oncorhynchus mykiss fish with the average weight of 121 ± 18 g [±SD] were allocated into four treatment groups: (1) control, (2) diazinon (0.1 mg L‑1), (3) vitamin C (300 mg kg‑1 in diet) plus diazinon (0.1 mg L-1) and (4) vitamin C (1000 mg kg-1 in diet) plus diazinon (0.1 mg L-1). After a period of two and four weeks, sampling was performed on their blood serums to measure Superoxide dismutase (SOD) and catalase (CAT) enzymes, total antioxidant capacity (TAC) and malondialdehyte (MDA) index. Findings indicated that the fish in diazinon treated group (without vitamin C in diet) showed significant (p<0.05) decrease in SOD and TAC enzyme activities after two weeks and also increase in CAT enzyme activity and MDA index in the end of fourth week in comparison with control group. The mentioned changes in antioxidant enzyme activity, total antioxidant capacity and MDA index could be attributed to destructive free oxiradicals such as superoxide and hydroxyl radicals produced in diazinon metabolism. In addition, based on our findings, a dose of 1000 mg of vitamin C in diazinon treated fish could cause significant decrease (P<0.05) or balance in CAT and SOD enzyme activity levels and also increase in TAC level and decrease in MDA index in comparison with the group without vitamin. On the other hand, a dose of 300 mg of vitamin C had no effect on decreasing SOD, CAT activity level, but could increase TAC level temporarily in the end of second week and also decrease MDA level in the end of fourth week. In this study, the antioxidant characteristic of vitamin C as a scavenger in removing free oxiradicals produced in diazinon metabolites; moderating antioxidant enzyme activity level, increasing total antioxidant capacity and also decreasing cellular damages caused by destructive lipid peroxidation process in presence of diazinon were reported. Regarding the results and considering the presence of agricultural contaminants, especially organophosphorous pesticides in domestic water sources which are the sources for fish farms, it seems necessary to add vitamin C to diet of cultured species in order to increase antioxidant defense and decrease the oxidative destructions.
Ali, M., Mirvaghefi, A., poorbagher, H., & Asadi, F. (2015). Antioxidant role of vitamin C on reducing oxidative stress induced subchronic toxicity of rainbow trout (Oncorhynchus mykiss) with diazinon. Journal of Animal Environment, 7(1), 73-87.
MLA
Mohsen Ali; Alireza Mirvaghefi; Hadi poorbagher; Farzad Asadi. "Antioxidant role of vitamin C on reducing oxidative stress induced subchronic toxicity of rainbow trout (Oncorhynchus mykiss) with diazinon". Journal of Animal Environment, 7, 1, 2015, 73-87.
HARVARD
Ali, M., Mirvaghefi, A., poorbagher, H., Asadi, F. (2015). 'Antioxidant role of vitamin C on reducing oxidative stress induced subchronic toxicity of rainbow trout (Oncorhynchus mykiss) with diazinon', Journal of Animal Environment, 7(1), pp. 73-87.
VANCOUVER
Ali, M., Mirvaghefi, A., poorbagher, H., Asadi, F. Antioxidant role of vitamin C on reducing oxidative stress induced subchronic toxicity of rainbow trout (Oncorhynchus mykiss) with diazinon. Journal of Animal Environment, 2015; 7(1): 73-87.
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