Protein profiling of Larak Island Cone snail venom by RP-HPLC

Document Type : Genetic

Authors

1 Islamic Azad University, Science and Research Branch, Faculty of Marine Science and Technology, Department of Marine Biology, Iran

2 Iran Pasteur Institute, Biotechnology Research Center, Venom & Toxin Laboratory, Iran,

3 Islamic Azad University, Science and Research Branch, Faculty of Marine Science and Technology, Department of Marine Chemistry, Iran

Abstract

Identification of venomous species of Persian Gulf cone snails and characterization of venom composition and their features is so important from the point of danger for underwater divers as well as medical importance. In this research, the specimens of Conus textile were collected off Larak Island from depth of 7 m. The collected samples were transferred to laboratory alive and were stored at -700C. The venom´s ducts were separated and homogenized with deionized water. The mixture centrifuged at 13000 rpm for 15 minutes at 4 ̊C. Supernatant was considered as extracted venom and stored at -20 C after lyophylization. The entity of venom determined by SDS-PAGE and RP-HPLC methods used to investigate the extracted venom and system to determine protein pattern. The results obtained with SDS-PAGE showed that proteins and peptides of venom were ranged between 6 to 250KD. Chromatogram of the venom demonstrated more than 44 large and small fractions.

Keywords

Journal of Animal Environment
Volume 5, Issue 1
May 2012
Pages 123-128

Files

Share

How to cite

Statistics