Cloning of the luxA and luxB genes of Vibrio fischeri

Document Type : Genetic

Authors

1 Department of Genetics, Faculty of Basic Sciences and Medicine, Islamic Azad University, Zanjan Branch

2 Cellular and molecular Biology Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Caspian Institute of Ecology, Sari, Iran

4 Department of Biotechnology, Faculty of Modern Medical Technologies, Cell and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran

5 Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran

6 Iranian Fisheries Science Research Institute, tehran, post box: 14155- 6116

Abstract

Bacterial bioluminescence is a phenomenon caused by luciferase enzymes. All of these enzymes catalyze the same chemical reactions and are heterodimers composed of an α and β subunit encoded on adjacent genes, luxA and luxB, together with genes luxCDE which code for a long-chain aliphatic aldehyde, will organize the vibrio fischeri lux operon. In the this study chromosomal fragments contain, luxA and luxB genes of the bacterium vibrio fischeri are amplified and subsequently the amplified fragments have cloned into pTZ57R vector. Cloning has confirmed by the PCR methods and digestion with restriction enzymes EcoRI / BamHI for luxA gene and BglII/KpnI for luxB gene. Results showed that sequenced luxA and luxB genes have 95% and 89% identity with the GenBank. Now luciferase cloned genes after subcloning into suitable expression vectors can be useful as reporters in different biotechnological and research fields.

Keywords

Journal of Animal Environment
Volume 3, Issue 2
July 2011
Pages 33-40

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