Molecular identification of Theileria ovis and Theileria lestoquardi among small ruminants of East Azerbaijan province during 2015 to 2016

Document Type : Genetic

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz University, Tabriz, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz University, Tabriz, Iran

4 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Theileria species are obligatory intracellular hemoprotozoan parasites transmitted by various species of hard ticks and cause Theleriosis. Geographically, the disease has a global distribution and is often occurred in the tropical and subtropical areas of the world. In Iran, the small ruminant Theleriosis is caused by Theileria ovis and T. lestoquardi (Hirci). This study was aimed to identify Theileria ovis and T. lestoquardi among the small ruminants of East Azerbaijan province based on molecular method. For this purpose, a total of 166 whole blood samples (including 125 sheep and 41 goats) were collected from different regions of East Azerbaijan province, and then, all samples were examined under routine microscopic method and molecular assay. According to the molecular findings, 30(18%) of 166 specimens were only infected with T. ovis, whereas no infection was found on microscopic examination. The infection was only related to sheep samples. There was no significant difference between infection and age and sex, statistically. Sequencing showed that the amplified fragments in the present study was 98-100% identical to the corresponding sequences on GeneBank. Phylogenetic analysis indicated that the sequence obtained in this study with many sequences from different parts of the world in different years in a set and is closely related to these sequences. As a result, it can be noted that Theileriosis has a clinical and subclinical feature among the small ruminants of the East Azarbaijan province and T.ovis is the main cause of Theileriosis.

Keywords

References

  1. Aktas, M.; Altay, K. and Dumanli, N., 2007. Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res, Vol. 100, No. 4, pp: 797-802.
  2. Aktas, M.; Altay, K. and Dumanli, N., 2005. Survey of Theileria parasites of sheep in eastern Turkey using polymerase chain reaction. Small Rumin Res, Vol. 60, No. 3, pp: 289-293.
  3. Alanazi, A.D.; Said, A.E.; Ghoneim, A.M.; Alyousif, M.S. and Alanazi, I.O., 2018. A comprehensive evaluation and first molecular report of Theileria ovis infection in small ruminants in Saudi Arabia. Trop Anim Health prod, DOI.org/10.1007/s11250-018-1663-y
  4. Altay, K.; Aktas, M. and Dumanli, N., 2007. Theileria infections in small ruminants in the East and Southeast Anatolia. Türkiye Parazitol Derg, Vol. 31, No. 4, pp: 268-271.
  5. Altay, K.; Dumanlia, N.; Holmanb, P.J. and Aktas, M., 2005. Detection of Theileria ovis in naturally infected sheep by nested PCR. Vet Parasitol, Vol. 127, No. 2, pp: 99-104.
  6. Durrani, A.Z.; Younus, M.; Kamal, N.; Mehmood, N. and Shakoori, A.R., 2011. Prevalence of Ovine Theileria Species in District Lahore, Pakistan. Pakistan J Zool, Vol. 43, No. 1, pp: 57-60.
  7. Foronda, P.; Casanova, J.C.; Valladares, B.; Martinez, E. and Feliu, C., 2004. Molecular systematics of several cyclophyllid families (Cestoda) based on the analysis of 18S ribosomal DNA gene sequences. Parasitol Res, Vol. 93, pp: 279-282
  8. Hadziavdic, K.; Lekang, K.; Lanzen, A.; Jonassen, I.; Thompson, E.M. and Troedsson, C., 2014. Characterization of the 18S rRNA Gene for Designing Universal Eukaryote Specific Primers. PLoS ONE Vol. 9, No. 2, e87624. https://doi.org/10.1371/journal.pone. 0087624.
  9. Hashemi-Fesharaki, R., 1997. Tick-borne diseases of sheep and goats and their related vectors in Iran. Parasitologia. Vol. 39, No. 2, pp: 115-117.
  10. Heidarpour Bami, M.; Haddadzadeh, H.R.; Kazemi, B.; Khazraiinia, P.; Bandehpour, M. and Aktas, M., 2009. Molecular identification of ovine Theileria species by a new PCR–RFLP method. Vet Parasitol, Vol. 161, No. 3-4, pp: 171-177.
  11. Heidarpour Bami, M.; Khazraiinia, P.; Haddadzadeh, H.R. and Kazemi, B., 2010. Identification of Theileria species in sheep in the eastern half of Iran using nested PCR RFLP and microscopic techniques. Iranian J Vet Res. Vol. 11, No. 3, pp: 262-266.
  12. Inci, A.; Ica, A.; Yildirim, A. and Duzlu, O., 2010. Identification of Babesia and Theileria species in small ruminants in Central Anatolia (Turkey) via reverse line blotting. Turkey Journal of Vetaraniary Animal Sciences. Vol. 34, No. 2, pp: 205-210.
  13. Iqbal, F.; Fatima, M.; Shahnawaz, S.; Naeem, M.; Shaikh, R.; Ali, M. and et al., 2011. A study on the determination of risk factors associated with babesiosis and prevalence of Babesia sp., by PCR amplification, in small ruminants from Southern Punjab (Pakistan). Parasite. Vol. 18, No. 3, pp: 229-234.
  14. Irshad, N.; Qayyum, M.; Hussain M. and Khan, Q., 2010. Prevalence of tick infestation and theileriosis in sheep and goats. Pakistan Vetaraniary Journal. Vol. 30, No. 3, pp: 178-180.
  15. Jalali, S.M.; Khaki, Z.; Kazemi, B.; Rahbari, S.; Shayan, P. and Bandehpour, M., 2014. Molecular Detection and Identification of Theileria Species by PCR-RFLP Method in Sheep from Ahvaz, Southern Iran. Iranian J Parasitol. Vol. 9, No. 1, pp: 99-106.
  16. Mohammadi, M.; Esmaeilnejad, B. and Jalilzadeh-Amin, Gh., 2017. Molecular detection, infection rate and vectors of Theileria lestoquardi in goats from West Azerbaijan province, Iran. VRF. Vol. 8, No. 2, pp: 139-144.
  17. Rashidi, A. and Razmi, G.R., 2013. Molecular detection of Theileria spp. in sheep and vector ticks in the North Khorasan Province, Iran. Trop Anim Health Prod. Vol. 45, No. 1, pp: 299-303.
  18. Razmi, G.R.; Eshrati, H. and Rashtibaf, M., 2006. Prevalence of Theileria spp. infection in sheep in South Khorasan province, Iran. Vet Parasitol. Vol. 140, No. 3-4, pp: 239-243.
  19. Razmi, G.R.; Pourhosseini, M.; Yaghfouri, S.; Rashidi, A. and Seidabadi, M., 2013. Molecular detection of Theileria spp. and Babesia spp. in sheep and Ixodid ticks from the northeast of Iran. J Parasitol. Vol. 99, No. 1, pp: 77-81.
  20. Riaz, M. and Tarawar, Z., 2017. Identification of Theileria species (Theileria ovis and Theileria lestoquardi) by PCR in apparently healthy small ruminants in and around Multan, southern PUNJAB, PAKISTAN. J Anim Plant Sci. Vol. 27, No. 3, pp: 809-818.
  21. Riaz, M.; Tasawar, Z. and Zaka Ullah, M., 2017. A survey on molecular prevalence, intensity and associated risk factors for ovine and caprine theileriosis from southern Punjab, Pakistan. Pakistan J Life Soc Sci. Vol. 15, No. 3, pp: 150-157.
  22. Soosaraei, M.; Motavalli Haghi, M.; Etemadifar, F.; Fakhar, M.; Hosseini Teshnizi, S.; Ziaei Hezarjaribi, H. and Asfaram, S., 2018. Status of theileriosis among herbivores in Iran: A systematic review and Meta-analysis. Vet World. Vol. 11, No. 3, pp: 332-341.
  23. Theodoropoulos, G.; Gazouli, M.; Ikonomopoulos, J.A.; Kantzoura, V. and Kominakis, A., 2006. Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification. Vet Parasitol. Vol. 135, No. 2, pp: 99-104.
  24. Uilenberg, G., 1995. International collaborative research: Significance of tick-borne hemoparasitic diseases to world animal health. Vet Parasitol. Vol. 57, No. 1-3, pp: 19-41.
  25. Zaeemi, M.; Haddadzadeh, H.; Khazraiinia, P.; Kazemi, B. and Bandehpour, M., 2011. Identification of different Theileriaspecies (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR–RFLP. Parasitol Res. Vol. 108, No. 4, pp: 837–843.
Journal of Animal Environment
Volume 12, Issue 1
February 2020
Pages 79-86

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