Genetic diversity of the two papulations of Common Cultured common carp (Cyprinus carpio) in guilan and Mazandran provinces using ten microsatellite markers

Document Type : Biodiversity

Authors

1 Department of Fisheries, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Fisheries and Aquaculture, Behdad Branch, Guilan Agricultural and Natural Resources Research and Training Center, Iran Agricultural Research, Education and Extension Organization, Rasht, Iran

Abstract

In this research to study genetic diversity of cultured broodstocks of common carp, 60 samples were collected from Guilan and Mazandran provinces (30 from each province). DNA were extracted by phonol- chloroform method and investigated for 11 microsatellites loci. The results showed that the range of alleles number, expected and observed heterozygocity were 11-18, 63%-86%, respective. The analysis of molecular variance was not showed high genetic diversity (93%) within populations, but the Fst and Rst values were significantly different between Guillan fish and Mazandran fish populations. The Fst value was 0.011 wich indicated the low genetic differentiation between the studied sites. this differentiation may be due to broodstock transportation by farmers. Tree investigated loci showed significant deviation from Hardy-Weinberg Equilibrium (p < 0.005), mosly due to the excess of heterozygocity of Mazandran samples. The Fst value and Gen flow due to number of alleles were 14.5 and 2.11, respectively. UPEMA cluster analysis based on Nei genetic distance showed that there are two different populations. Based on the results of this study it can be concluded that there were significant differences between number of allelesand genetic diversity in Guilan and Mazandran.
 

Keywords

References

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Journal of Animal Environment
Volume 12, Issue 1
February 2020
Pages 285-292

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