The effect of pure microcystin (microcystin-RR) from Microcystis aeroginosa (Kützing, 1846) on the survival of Daphnia magna (Straus, 1820) and determination of its lethal concentration (LC50-96h)

Document Type : Animal environment

Authors

Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

This study was carried out to investigate the effect of Microcystin-RR (MC-RR) extracted from Microcystis aeroginosa microalgae on Daphnia magna and determine the lethal concentration of this toxin (LC50-96h). Twenty-one Daphnia magna were exposed to concentrations of 0.5, 2, 10, 20 and 50 μg.ml-1 of MC-RR Microcystin in each replicate, and the daphnia survival for 24 to 96 hours was calculated. One treatment was considered as control (no toxin), each treatment had 3 replications. According to the results of this study, microcystin (MC-RR) had a lethal effect on Daphnia magna, so that even at a low concentration (concentration of 0.5), about 38% of mortality was observed, and none of the Daphnia magna had the ability to survive at 2 μg.ml-1 of pure MC-RR Microcystin for 96 hours. This ability decreased by increasing the concentration of poison, so that at the concentration of 10 μg.ml-1 of Microcystin, on the second day and at concentrations of 20 and 50 μg.ml-1 of poison, all daphnia were dead on the first day. The results of this study showed that in the concentration of 1.75 μg.ml-1 MC-RR Microcystin, during 96 hours, 99 percent of daphnia were died.

Keywords

References

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Journal of Animal Environment
Volume 12, Issue 1
February 2020
Pages 361-368

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