Evaluation of the Antibacterial Effects of Iron Oxide-Alginate Nanoparticles on Formation of Pseudomonas aeruginosa Biofilm

Document Type : Disease

Authors

1 Department of Genetics and Biotechnology, Faculty of Biological Science, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran

2 Department of Biochemistry and Biophysics, Faculty of Biological Science, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran

Abstract

Pseudomonas aeruginosa, is one of the important causes of nosocomial infections. Unfortunately, this bacterium has very high level of antibiotic resistance due to the presence of resistance genes, the efflux pumps and the formation of biofilms. The aim of this study was to investigate the inhibitory effect of magnetite-alginate nanoparticles (M-AlgNPs) on P. aeruginosa biofilm. M-AlgNPs were synthesized utilizing a coprecipitation method. The structural properties of the synthesized nanoparticles were investigated by using Fourier Transform Infrared (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) and X-ray Diffraction (XRD). Antibacterial activity was tested by determining the minimum inhibitory concentration (MIC) using microdilution method and biofilm formation was measured using microtiter plate tests. M-AlgNPs at a concentration of 0.256 to 90 µg/ml inhibited biofilm formation in clinical strains of Pseudomonas. The present investigation revealed that application of M-AlgNPs as antibacterial agents may be effective in inhibiting biofilm formation of P. aeruginosa.

Keywords

References

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Journal of Animal Environment
Volume 12, Issue 3
June 2020
Pages 501-510

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