The Nucleotide Variation of the Iranian Cave Fish (Iranocypris Typhlops) Using 12S rRNA Gene Sequencing

Document Type : Biodiversity

Authors

1 Department of Biology, Faculty of Biological Sciences, Islamic Azad University Varamin-Pishva Branch, Pishva, Iran

2 Department of Genetic and Biotechnology, Faculty of Biological Sciences, Islamic Azad University Varamin-Pishva Branch, Pishva, Iran

3 Department Of Environment expert, Tehran, Iran

Abstract

The Iranian Cave Fish (Iranocypris typhlops) is one of at risk species and important genetic resource that only habitat of this species is a cave in Lorestan province. In order to Genetic Determination of Iranian Cave Fish, after sampling and DNA extraction from ventral fins of 2 fish, the 12S rRNA region was used. After loci amplifying in PCR, the all samples were sequenced. The results revealed that all sequence pattern of 12S rRNA region were similar that it can be concluded that The 12S rRNA region in the two Iranian Cave Fish were a one haplotype and there weren’t any variation and it would appear that the high genetic similarities and no differences are in the two Iranian Cave Fish of this population. 

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