Effect of Silybum marianum extract on electrocardiographic parameters in pekin ducks with lead nitrate poisoning

Document Type : (original research)

Authors

1 Department of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Animal Science, Garmsar Branch, Islamic Azad University, Garmsar, Iran

Abstract

Lead is the second most dangerous heavy metal, which is widely spread in the environment due to the high use of this metal in industry, and through poisoning of water and food, it can poison humans and animals. The aim of this study was to investigate the effect of Silybum marianum seed extract on electrocardiogram parameters in lead poisoned ducks. 90 pekin ducks were divided into 3 groups including healthy control group, poisoned control group (40 mg/kg lead nitrate in the diet) and poisoned treatment group (40 mg/kg lead nitrate and 300 mg/kg of plant extract in the diet). In order to measure the height of T, S and R waves and the distances of ST, RR, QT and QRS and the electrical axis of the heart at the ages of 28 and 60 days, an electrocardiogram was prepared from 8 ducks in each group. The results showed that changes in R, S and T waves were observed in the poisoned control group and the height of these waves increased compared to the healthy control group, which is the result of lead-induced hypertension. The plant extract in the treatment group prevented the abnormal increase of these waves and the decrease of height in R wave in derivation III and also T wave in derivation III and aVF at 60 days were significant, statistically. Also, QRS and QT intervals increased in the poisoned control group and the plant extract significantly prevented the increase in QRS interval of derivation II at 60 days, QT interval in derivation III at 28 days and in aVF derivation at 60 days. Therefore, the seed extract improved cardiac electrocardiogram indices in lead-poisoned ducks.

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  1. DerMarderosian, A. and Louis, P., 2001. The review of natural products. 1st ed. Facts and Comparisons. St. pp: 405-409.
  2. Dey, S.; Swarup, D. and Singh, G.R., 1993. Effect of experimental lead toxicity on cardiovascular function in calves. Vet Hum Toxicol. Vol. 35, pp: 501-503.
  3. Kamil, K.A.; Kartasudjana, R.; Iskandar, S. and Latipudin, D., 2011. Effect of phytate in diet and lead in drinking water on blood mineral and growth of ducks. International Scientific Symposium-60 Years of Animal Science Higher Education in Moldova.
  4. Kirby, Y.K. and Wideman, R.F.R., 1995. Molecular genetics analysis of loci affecting resistance or susceptibility to pulmonary hypertension syndrome. Poultry Sci. Vol. 74, No. 1, pp: 91.
  5. Kober, T. and Cooper, G., 1976. Lead competitively inhibits calcium dependent synaptic transmission in the bullfrog sympathetic ganglion. Nature. Vol. 262, pp: 704-705.
  6. Kopp, S.; Baker, J.; LS, D.A. and Hawley, P.L., 1978. Simultaneous recording of Hisbund le electrogram, electrocardiogram, and systolic tension from intact modified Langendorff rat heart preparations. I: effects of perfusion time, cadmium, and lead. Toxicol Appl Pharmacol. Vol. 46, pp: 475-487.
  7. Kopp, S.J.; Perry, M.Jr.; Glonek, T.; Erlanger, M.; Perry, E.F. and Barany, M., 1980. Cardiac physiologic-metabolic changes after chronic low-level heavy metal feeding. Am J Physiol. Vol. 239, pp: H22-H30.
  8. Lumeii, J.T., 1985. Clinico-pathologic aspects of lead poisoning of birds: A review. The Vet Quarterly. Vol. 7, No. 2, pp: 133-138.
  9. Mortinez, A.; Jeffrey, S. and Odom, T.W., 1997. Electrocardiographic diagnosis of cardiomypathies in aves. Poult Avian Boil Rev. Vol. 8, pp: 9-20.
  10. Myerburg, R., 1997. Sudden cardiac death in persons with normal (or near normal) hearts. Am J Cardiol. Vol. 79, pp: 3-9.
  11. Myerson, R. and Eisenhauer, J., 1963. Atrio-ventricular conduction defects in lead poisoning. Am J Cardiol, Vol. 11, pp: 409-412.
  12. Navas-Acien, A.; Guallar, E.; Silbergeld, E.K. and Rothenberg, S.J., 2007. Lead exposure and cardiovascular disease - A systematic review. Environ Health Perspect. Vol. 115, pp: 472-782.
  13. Narayana, K. and Al-Bader, M., 2011. Ultrastructural and DNA damaging effects of lead nitrate in the liver. Exp &Tox Path. Vol. 63, pp: 43-51.
  14. Nosratola, D. and Vaziri, K., 2008. Mechanisms of lead induced hypertension and cardiovascular disease. Am J Physiol Heart Circ Physiol. Vol. 295, pp: H454-H465.
  15. Silbergeld, E.; Fales, J. and Goldberg, A., 1974. Evidence for a junctional effect of lead on neuromuscular function. Nature, Vol. 247, pp: 49-50.
  16. Silver, W. and Rodriguez-Torres R., 1968. Electrocardiographic studies in children with lead poisoning. Pediatrics. Vol. 41, pp: 1124-1127.
  17. Sunil, K.M.; Asok, M.; Nguyen, T.V. and Bharat, B.A., 1999. Silymarin suppresses TNF induced activation of NFKb, c-Jun N-Terminal Kinase, and Apoptosis. J Molecul Biol. Vol. 18, pp: 6800-6809.
  18. Tuormaa, T.E., 1995. The adverse effects of lead. J Orthomol Med. Vol. 10, No. 3-4, pp: 149-164.
  19. Read, J. and Williams, J., 1952. Lead myocarditis: report of a case. Am Heart J. Vol. 15, pp: 797-802.