Comparative Study of Different Levels of Sodium Di-formate (NDF) and Formic Acidifiers on Nutrition, Growth and Activity of Digestive Enzymes in beluga (Huso huso)

Document Type : (original research)

Authors

1 Department of Fisheries, Faculty of Agriculture and Natural Resources, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran

2 Deputy of Clinical Sciences, Faculty of Veterinary Medicine. Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Recently, the use of Acidifier (a group of organic acids) has been increased in aquaculture. The purpose of this study is to evaluate the effects of Acidifier (sodium diformate and Formic acid) on the growth performance, nutrition indices, and Digestive enzymes activity of the juvenile Huso huso. A total of 420 juvenile Huso huso (30.55 ± 1.72 g) was randomly divided into seven experimental treatments (Three replicates each). All groups were fed with the experimental diet for 60 days. The Bioassay conducted on the first day and after 30 and 60 days and then growth parameters were investigated. The experimental diets contain 0.05, 0.1 and 0.15 % sodium diformate (NDF) and 0.05, 0.1 and 0.15 % Formic acid (Formi). diet control was without any acidifier. Results show that all growth and Intestinal enzymes (trypsin, chymotrypsin and alkaline phosphatase) are affected by acidifier and in these two parameters  groups that fed 0.1 % supplemented food by NDF and citric acid compared to the control group. The best FCR and SGR observed in the groups fed with 0.1 % supplemented food by NDF)0.5±00), (0.5±0.005) (p < 0.05) at day 30 respectively. Although the best FCR and SGR observed in the groups fed with 0.1 % supplemented food by Formic acid (0.81±0.001), (2.7±0.005) (p < 0.05) at day 60 respectively. Intestinal enzymes (trypsin, chymotrypsin and alkaline phosphatase) are affected by acidifier. The highest activity of trypsin and chymotrypsin was significant in groups 0.1 % supplemented food by NDF (P˂0.05) in  the first 30 days. Alkaline phosphatase activity increased with increasing acidifier diets in groups, but this increase was not significant compared to the control (p>0.05). It can be concluded that the addition of  0.1% of NDF to beluga diets improves growth parameters and decreases FCR, increases digestive enzyme activity. Moreover, these results indicated that 0.1 % of NDF and citric acid in the diet could be a useful food supplement preferably at 30 days and can be used to improve the growth parameters in juvenile Huso huso.

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References

  1. Ahmadnia, H.R.; Motlag, H.R.; Farhangi, M.; Rafiee, Gh. and Noori, F., 2012. Modulating gut microbiota and digestive enzyme activities of Artemia urmiana by administration of different levels of (Bacillus subtilisand) and (Bacillus licheniformis). Aquaculture International. Vol. 20, No. 4, pp: 693-705. 
  2. Castillo, A.; Sanchez, C.; Dominguez, J.; Kaattari, S.L. and Villena, A.J., 1993. Ontogeny of IgM and IgM-bearing cells in (rainbow trout). Developmental and Comparative Im­munology. Vol. 17, No. 5, pp: 419-424.
  3. Castillo, S.; Rosales, M.; Pohlenz, M. and Gatlin, D.M., 2014. Effects of organic acids on growth performance and digestive enzyme activities of juvenile red drum (Sciaenops ocellatus). Aquaculture. Vol. 433, pp: 6-12.
  4. Ebrahimi, M.; Daeman, N.H.; Chong, C.M.; Karami, A.; Kumar, V.; Hoseinifar, S.H. and Romano, N., 2017. Comparing the effects of different dietary organic acids on the growth, intestinal short-chain fatty acids, and liver histopathology of red hybrid tilapia (Oreochromis sp.) and potential use of these as preservatives. Fish Physiology and Biochemistry. Vol. 43, No. 4, pp:1195-1207.
  5. Erlanger, B.F.; Kokowsky, N. and Cohen, W., 1961. The preparation and properties of two new chromogenic substrates of trypsin. Archives of Biochemistry and Biophysics. Vol. 95, No. 2, pp: 271-278.
  6. Hardy, R.W., 2000. New developments in aquatic feed ingredient, and potential of enzyme supplements. A vances en Nutricion Aquicola V. memorias, V. Simposium Internacional de nutricion Acuicola. 19-22 Noviembre Merdia, Yucatan, Mexico. pp: 216-227.
  7. Hassaan,M.S.; Wafa, M.A.; Soltan, M.A.; Goda, A.S. and Mogheth, N.M.A., 2014. Effect of Dietary Organic Salts on Growth, Nutrient Digestibility, Mineral Absorption and Some Biochemical Indices of Nile Tilapia; Oreochromis niloticus L. Fingerlings. World Applied Sciences Journal Vol. 29, No. 1, pp: 47-55.
  8. Hoseinifar, S.H.; Sun, Y.Z. and Caipang, C.M., 2017. Short chain fatty acids as feed supplements for sustainable aquaculture: an updated view. Aquaculture Research. Vol. 48, No. 2, pp:1380-1391.
  9. Hoseinifar, S.H.; Esteban, M.Á.; Cuesta, A.  and Sun, Z., 2015. Prebiotics and fish immune response: a review of current knowledge and future perspectives. Reviews in Fisheries Science & Aquaculture. Vol. 23, pp: 315-328.
  10. Kuzmina, V.V.; Shekovtsova, N. and Bolobonina, V., 2010. Activity dynamics of proteinases and glycosidases of fish chyme with exposure in fresh and brackish water. Biology Bulletin. Vol. 37, No. 6, pp: 605-611.
  11. Li, W.F.; Feng, J.; Xu, Z.R. and Yang, C.M., 2004. Effects of non-starch polysacarides enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley. World Journal of Gastroenterology. Vol. 10, No. 6, pp: 856-890.
  12. Liebert, F.; Mohamed, K. and Lückstädt, C., 2010. Effects of diformates on growth and feed utilization of all male Nile Tilapia fingerlings (Oreochromis niloticus) reared in tank culture.  XIV International Symposium on Fish Nutrition and Feeding, Qingdao, China, Book of Abstracts. 190 p.
  13. Lim, C.; Lückstädt, C.; Webster, C.D. and Kesius, P., 2015. Organic acids and their salts (chapter 15) In: Lee, C.S.; Lim, C.; Gatlin, D.M. and Webster, C.D., (Eds.), Dietary Nutrients, Additives and Fish Health. Wiley-Blackwell.
    pp: 305-315.
  14. Luckstadt, C., 2008. The use of acidifiers in fish nutrition. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. Vol. 3, pp: 1-8.
  15. Mohammadian, T.; Alishahi, M.; Tabandeh, M.; Ghorbanpoor, M. and  Gharibi, D., 2017. Effect of Lactobacillus plantarum and Lactobacillus delbrueckii subsp. bulgaricus on growth performance, gut microbial flora and digestive enzymes activities in Tor grypus (Karaman, 1971). Iranian Journal of Fisheries Sciences. Vol. 16, No. 1, pp: 296-317.
  16. Morken, T.; Kraugerud, O.F.; Barrows, F.T.; Sorensen, M.; Storebakken, T. and Overland, M., 2011. Sodium diformate and extrusion temperature affect nutrient digestibility and physical quality of diets with fish meal and barley protein concentrate for rainbow trout (Oncorhynchus mykiss). Aquaculture. Vol. 317, pp :138-145.
  17. Ng, W.K.; Koh, C.H.; Teoh, C.Y. and Romano, N., 2015. Farm-raised tiger shrimp (Penaeus monodon) fed commercial feeds with added organic acids showed enhanced nutrient utilization. Aquaculture Resources. Vol. 449, pp: 69-77.
  18. Romano, N.; Koh, C.B. and Ng, W.K., 2015. Dietary microencapsulated organic acids blend enhances growth, phosphorus utilization, immune response, hepatopancreatic integrity and resistance against Vibrio harveyi in white shrimp, Litopenaeus vannamei. Aquaculture. Vol. 435, pp: 228-236.
  19. Rungruangsak, T.K.; Rustad, A.; Sunde, J.; Eiane, S.A.; Jensen, H.B.; Opstvedt, J.; Nygård, E.; Samuelsen, T.A.; Mundheim, H. and Luzzana, U., 2002. In vitro digestibility based on fish crude enzyme extract for prediction of feed quality in growth trials. Journal of the Science of Food and Agriculture. Vol. 82, No. 6, pp: 644-654.
  20. Silva, B.C.; Nolasco, S.H.; Magallón Barajas, F.; Civera Cerecedo, R.; Casillas Hernández, R. and Seiffert, W., 2015.  Improved digestion and initial performance of white leg shrimp using organic salt supplements. Aquac.Nutr. Vol. 22, No. 5, pp: 997-1005.
  21. Zhu, Y.; Qiu, X.; Ding, Q.; Duan, M. and Wang, C., 2014. Combined effects of dietary phytase and organic acid on growth and phosphorus utilization of juvenile yellow catfish (Pelteobagrus fulvidraco). Aquaculture. Vol. 430, pp: 1-8.
Journal of Animal Environment
Volume 13, Issue 1
May 2021
Pages 247-258

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