Effect of aqueous extract of garlic (Allium sativum) in the treatment of Ichthyophthirius in goldfish (Carassius auratus)

Document Type : (original research)

Authors

Department of health and aquatic diseases, Faculty of veterinary medicine, Sanandaj branch, Islamic Azad University, Sannadaj, Iran

Abstract

Ictiophytosis is one of the most important freshwater fish diseases. In this study, the therapeutic effect of aqueous extract of garlic in treatment of Goldfish was investigated. A group of 200 apparently healthy goldfish with weigh 15g were randomly divided into five treatments group with three replications. Extract obtained from garlic was obtained by direct and indirect methods at 177 and 570 ppm concentrations. In long-term bath was tested on fish infected with parasites. During the experiment, which continued until the fish's body was completely cleaned, the parasites were counted once every 24 hours with a wet mount method. The results showed that the extract had a significant effect on the number of parasites and disease treatment in different groups. In other words, in cure during the number of visible parasites on the fish's body controls was significantly (p < 0.05) was observed in the parasites on the fish groups were further tested. The extract obtained from the Buchmann’s methods on both the concentration of the extract obtained by Penea’s Method was more effective (p < 0.05) and this can be an effective herbal remedy used to treat disease in Gold Fish.

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  1. Buchmanna, K., Jensena, P. B., and Krusea, K. D., 2003. Effects of Sodium Percarbonate and Garlic Extract on Ichthyophthirius multifiliis Theronts and Tomocysts: In Vitro Experiments; North American Journal of Aquaculture. Vol. 65, No. 1, pp: 21-24.
  2. Colorni, A., Avtalion, R., Knibb, W.  Berge, E., Colorni, B. and Timan, B., 1998. Histopathology of sea bass Dicentrarchus labrax experimentally infected with Mycobacterium marinum and treated with streptomycin and garlic (Allium sativum) extract. Aquac. Vol. 190, pp: 1-17.
  3. Corzo-Martinez, M.; Corzo, N. and Villamiel, M., 2007. Biological properties of onions and garlic, Trends in. Food Sci. and Techno. Vol. 18, pp: 609-625.
  4. Giannenas, I.; Triantafillou, E.; Stavrakakis, S.; Margaroni, M.; Mavridis, S.; Steiner, T. and Karagouni, E., 2012. Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss). Aquaculture. Vol. 353, pp: 26-32.
  5. Konjufca, V.H.; Pesti, G.M. and Bakalli, R.I., 1997. Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poul. Sci. Vol. 76, pp: 1264-1271.
  6. Pena, N.; Auro, A. and Sumano, H., 1988.  A comparative trial of garlic, its extract and ammonium- potassium tartrate as anthelmintics in carp. Journal-of-Ethnopharmacology. Vol. 24, No. 2-3, pp: 199-203
  7. Peyghan, R.; Powel, M.D. and Zadkarami, M.R., 2008. In vitro effect of Garlic extract and Metronidazole against Neoparamoeba pemaquidensis and isolated amoeba from atlantic salmon. Pakistan jornal of Biological sciences. Vol. 11, No. 1, pp: 41-47.
  8. Ramos, A., 1991. Treatment of saprolegniasis in rain trout with garlic. Veterinaria Mexico. Vol. 22, No. 1, pp: 98.
  9. Sahandi, J.; Gholipour-Kanani; H. and Taheri, A., 2012. Influence of Garlic (Allium sativum) and Mother worth (Matricaria chamomilla) Extract on Ichthyophtirius multifilus Parasite Treatment in Sail Fin Molly (Poecilia latipinna) Ornamental Fish. 2nd International Conference on Asia Agriculture and Animal (ICAAA 2012).
  10. Sinai, T. and Zilberg, D., 2014. Efficacy of garlic based treatments against monogenean parasites infecting the guppy, Vet Parasitol.  Jun 16. Vol. 203, No. 1-2, pp: 51-58. doi: 10.1016/j.vetpar.2014.02.002.
  11. Sivam, G.P., 2001. Recent advances on the nutrition effects associated with the use of garlic as supplement. Am. Soc. Nutr. Sci. Vol. 31, pp: 955-962.
  12. Slusarenko, A.J.; Patel, A. and Portz. D., 2008. Control of plant diseases by natural products: Allicin from garlic as a case study. European J. Plant Pathol. Vol. 12, No. 3, pp: 313-322.
  13. Soko, C.K. and Barker, D.E., 2004. Efficacy of crushed garlic and lemon juice as bio-product treatments for Ichthyophthirius multifiliis infections among juvenile Nile tilapia, Oreochromis niloticus. Aquaculture Associaton of Canada Special Publication. Vol. 9, pp: 108-110.
  14. Wang, H. and Nq, T., 2010. The rolor of lysozymes. Biochemistry Reserch Community. Vol. 336, No. 1, pp: 100-104.
  15. William, Q., 2008. Least toxic controls of plant diseases. Brooklyn Botanic garden. Natural Disease Control. Vol. 11, 225 p.