Primary study of genetic structure of Jinga shrimp (Metapenaeus affinis) population in northern Persian Gulf waters by 16S rRNA gene sequencing

Document Type : Biodiversity

Authors

1 Department of Fisheries, Faculty of Marine and Atmospheric Science and Technology, University of Hormozgan, P.O.Box: 3995, Bandar Abbas, Iran

2 Department of Genetics, Persian Gulf and Oman Sea Research Center, P.O.Box: 1597, Bandar Abbas, Iran

Abstract

Regarding the importance of Jinga shrimp (Metapenaeus affinis) in shrimp catch cycle in the Persian Gulf, primary study of genetic structure of this species population was considered by sequencing of mitochondrial 16S rRNA gene. A number of 18 shrimps were collected from the regions of Bandar Abbas, Bushehr and Khuzestan and after DNA extraction using phenol-chloroform method, the PCR for amplification of 16S rRNA was optimized. The maximum amount of F- statistic parameter was 0.750 calculated between samples of Bandar Abbas and Khuzestan and the minimum amount was -0.105 between the samples of Bushehr and Khuzestan. At probability level of 0.05, population differentiation was significant between Bandar Abbas and two other regions of Bushehr and Khuzestan but not significant between the regions of Bushehr and Khuzestan, which was confirmed by test of exact p values within populations. Mean values of Tajima’s D and Fu’s Fs between the regions for the 18 sequences were -0.823 and 1.181, respectively that were not statistically significant. Phylogenetic trees showed the differentiation of Bandar Abbas population from the two other regions but this hypothesis needs supplementary investigations with more samples as well as by analyzing other mitochondrial and genomic markers.

Keywords

Journal of Animal Environment
Volume 7, Issue 3 - Serial Number 3
November 2015
Pages 147-154

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