Invitro antifungal activity of Nettle (Urtica dioica) and Basil (Ocimum basilicum) extracts on Saprolegnia parasitica

Document Type : Disease

Authors

Department of Fishery, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, P.O.Box: 578, Sari, Iran

Abstract

Fungal infections are one of the main factors responsible for mortality and economic loss to the fish industry in the world. In this study, antifungal effect of Basil (Ocimum basilicum) and Nettle (Urtica dioica) aqueous and ethanolic extracts against Saprolegnia parasitica was investigated. Basil and Nettle aqueous and ethanolic extracts were prepared in 5 dosages (0, 250, 350, 700 and 1050 mg L-1) and mixed with Sabroud Dextrose Agar medium in five replicate.  Then, the inhibition of S. parasitica mycelia growth was measured in different dosages. The result of this study showed that among of experimental extracts, the Nettle aqueous extract (250 mg L-1) performed more effectively compared to other extracts for the growth inhibition of S. parasitica (p<0.05). The present study revealed that the Nettle aqueous extract at a concentration of 250 mg L-1 can be used as a treatment for saprolegniosis. 

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