Study the molecular prevalence rate of Toxoplasma gondii in raw milk samples collected from Alborz province by PCR method

Document Type : (original research)

Authors

1 Department of Food Hygiene, Faculty of Veterinary, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Clinical Care and Health Promotion Research Center, Karaj branch, Islamic Azad University, Karaj, Iran

3 Department of parasitology, Faculty of Veterinary, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

Consumption of good unheated food of animal origin is one of the main causes of Toxoplasmosis. The present study was performed to evaluate the prevalence of Toxoplasma gondii in raw milk samples collected from Alborz province. 350 samples of raw milk of cows, sheep and goats were collected and transferred to the laboratory. DNA extraction was performed from the samples and Toxoplasma gondii B1 gene was detected using a polymerase chain reaction. 30 samples out of 350 samples of raw milk evaluated contained Toxoplasma gondii B1 gene (8.57%). The molecular prevalence of Toxoplasma gondii in raw milk samples of cattle, sheep and goats was (5.33, 12.00 and 10) percent, respectively. Raw and unpasteurized sheep milk is considered to be the highest risk source for Toxoplasma gondii transmission. Boiling raw milk and using pasteurized milk is recommended to reduce the risk of Toxoplasma gondii.

Keywords

Main Subjects

References

  1. Amairia, S.; Rouatbi, M.; Rjeibi, M.R.; Nouasri, H.; Sassi, L.; Mhadhbi, M. and Gharbi, M., 2016. Molecular prevalence of Toxoplasma gondii DNA in goats’ milk and seroprevalence in Northwest Tunisia. Veterinary Medicine and Science. Vol. 2, pp: 154-160.
  2. Asgari Nasab, M.; Mortazavi, A.; Namazi, J. and Kazemi, S., 2011. Identification of the B1 gene of Toxoplasma gondii in heart, liver, brain and muscles of cow and sheep by PCR. . 20TH National Conference on Food science and technology.
  3. Belluco, S.; Simonato, G.; Mancin, M.; Pietrobelli, M. and Ricci, A., 2018. Toxoplasma gondii infection and food consumption: a systematic review and meta-analysis of case-controlled studies. Critical Reviews in Food Science and Nutrition. Vol. 58, pp: 3085-3096.
  4. Boughattas, S., 2017. Toxoplasma infection and milk consumption: Meta-analysis of assumptions and evidences. Critical Reviews in Food Science and Nutrition. Vol. 57, pp: 2924-2933.
  5. Costa, V. and Langoni, H., 2010. Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats. Journal of Venomous Animals and Toxins including Tropical Diseases. Vol. 16, pp: 368-374.
  6. Daryayi, A.; Sharif, M.; Lakterashi, B.; Ziyayi, H. and Ajmi, A., 2006. Toxoplasma gondii infection in livestock. J Mazandaran Univ Med Sci. Vol. 16, pp: 60-66.
  7. Dubey, J., 1995. Serologic prevalence of toxoplasmosis in cattle, sheep, goats, pigs, bison, and elk in Montana. Journal of the American Veterinary Medical Association. Vol. 186, pp: 969-970.
  8. Dubey, J., 1998. Advances in the life cycle of Toxoplasma gondii. International journal for parasitology. Vol. 28, pp: 1019-1024.
  9. Dubey, J.; Sundar, N.; Hill, D.; Velmurugan, G.; Bandini, L.; Kwok, O.; Majumdar, D. and Su, C., 2008. High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA. International journal for parasitology. Vol. 38, pp: 999-1006.
  10. Dubey, J.; Verma, S.; Ferreira, L.; Oliveira, S.; Cassinelli, A.; Ying, Y.; Kwok, O.; Tuo, W.; Chiesa, O. and Jones, J., 2014. Detection and survival of Toxoplasma gondii in milk and cheese from experimentally infected goats. Journal of food protection. Vol. 77, pp: 1747-1753.
  11. Grigg, M.E. and Boothroyd, J.C., 2001. Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene. Journal of clinical microbiology. Vol. 39, pp: 398-400.
  12. Guo, M.; Dubey, J.P.; Hill, D.; Buchanan, R.L.; Gamble, H.R.; Jones, J.L. and Pradhan, A.K., 2015. Prevalence and risk factors for Toxoplasma gondii infection in meat animals and meat products destined for human consumption. Journal of food protection. Vol. 78, pp: 457-476.
  13. Hamidinejat, H.; Goraninejad, S.; Ghorbanpoor, M.; Nabavi, L. and Akbarnejad, F., 2008. Role of Toxoplasma gondii in abortion of ewes in Ahvaz (South-West Iran). Bull Vet Inst Pulawy. Vol. 52, pp: 369-371.
  14. Hill, D.E.; Chirukandoth, S. and Dubey, J.P., 2005. Biology and epidemiology of Toxoplasma gondii in man and animals. Animal health research reviews. Vol. 6, pp: 41-61.
  15. Hove, T.; Lind, P. and Mukaratirwa, S., 2005. Seroprevalence of Toxoplasma gondii infection in goats and sheep in Zimbabwe. Onderstepoort Journal of Veterinary Research. Vol. 72, pp: 267-272.
  16. Hutchison, W., 1965. Experimental transmission of Toxoplasma gondii. Nature. Vol. 206, pp: 961-962.
  17. Inpankaew, T.; Pinyopanuwut, N.; Chimnoi, W.; Kengradomkit, C.; Sununta, C.; Zhang, G.; Nishikawa, Y.; Igarashi, I.; Xuan, X. and Jittapalapong, S., 2010. Serodiagnosis of Toxoplasma gondii infection in dairy cows in Thailand. Transboundary and emerging diseases. Vol. 57, pp: 42-45.
  18. Jones, J.L.; Kruszon-Moran, D.; Wilson, M.; McQuillan, G.; Navin, T. and McAuley, J.B., 2001. Toxoplasma gondii infection in the United States: seroprevalence and risk factors. American journal of epidemiology. Vol. 154, pp: 357-365.
  19. Klun, I.; Djurković‐Djaković, O. and Thulliez, P., 2007. Comparison of a commercial ELISA with the modified agglutination test for the detection of Toxoplasma gondii infection in naturally exposed sheep. Zoonoses and public health. Vol. 54, pp: 165-168.
  20. Lass, A.; Pietkiewicz, H.; Szostakowska, B. and Myjak, P., 2012. The first detection of Toxoplasma gondii DNA in environmental fruits and vegetables samples. European journal of clinical microbiology & infectious diseases. Vol. 31, pp: 1101-1108.
  21. Liu, Q.; Wang, Z.D.; Huang, S.Y. and Zhu, X.Q., 2015. Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. Parasites & vectors. Vol. 8, pp: 1-14.
  22. Mostafavi, S. and Jalali Monfared, L., 2012. Toxoplasmosis epidemiology in Iran: a systematic review. J Isfahan Med School. Vol. 176, pp: 1-15.
  23. Negash, T.; Tilahun, G.; Patton, S.; Prevot, F. and Dorchies, P.,2004. Serological survey on toxoplasmosis in sheep and goats in Nazareth, Ethiopia. Revue de medecine veterinaire. Vol. 155, pp: 486-488.
  24. Ossani, R.A.; Borges, H.A.T.; Souza, A.P.; Sartor, A.A.; Miletti, L.C.; Federle, M. and Moura, A.B., 2017. Toxoplasma in milk of naturally infected dairy ewes on west Mesiregion of santa catarina State , Brazil. Arg. Bras. Med. Vet. Zoote C. Vol. 69, pp: 1294-1300.
  25. Powell, C.C.; Brewer, M. and Lappin, M.R., 2001. Detection of Toxoplasma gondii in the milk of experimentally infected lactating cats. Veterinary parasitology. Vol. 102, pp: 29-33.
  26. Sabin, A.B., 1939. Biological and immunological identity of Toxoplasma of animal and human origin. Proceedings of the Society for Experimental Biology and Medicine. Vol. 41, pp: 75-80.
  27. Sevgili, M. and Babur, C., 2005. Determination of seropositivity for Toxoplasma gondii in sheep in Sanliurfa province. Turk. J. Vet. Anim. Sci. Vol. 29, pp: 107-111.
  28. Shaapan, R.; El-Nawawi, F. and Tawfik, M., 2008. Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep. Veterinary Parasitology. Vol. 153, pp:
    359-362.
  29. Spišák, F.; Turčeková, Ľ.; Reiterová, K.; Špilovská, S. and Dubinský, P., 2010. Prevalence estimation and genotypization of Toxoplasma gondii in goats. Biologia. Vol. 65, pp: 670-674.
  30. Tavassoli, M.; Esmaeilnejad, B.; Malekifard, F.; Soleimanzadeh, A. and Dilmaghani, M., 2013. Detection of Toxoplasma gondii DNA in Sheep and Goat Milk in Northwest of Iran by PCR-RFLP. Jundishapur Journal of Microbiology. Vol. 6, pp: 189-198.
  31. Tenter, A.M.; Heckeroth, A.R. and Weiss, L.M., 2000. Toxoplasma gondii: from animals to humans. International journal for parasitology. Vol. 30, pp: 1217-1258.
  32. Van der Puije, W.; Bosompem, K.; Canacoo, E.; Wastling, J. and Akanmori, B., 2000. The prevalence of anti-Toxoplasma gondii antibodies in Ghanaian sheep and goats. Acta tropica. Vol. 76, pp: 21-26.
  33. Zhou, D.H.; Zhao, F.R.; Lu, P.; Xia, H.Y.; Xu, M.J.; Yuan, L.G.; Yan, C.; Huang, S.Y.; Li, S.J. and Zhu, X.Q., 2012. Seroprevalence of Toxoplasma gondii infection in dairy cattle in southern China. Parasites & vectors. Vol. 5, pp: 1-4.
  34.  
Journal of Animal Environment
Volume 13, Issue 3
October 2021
Pages 41-48

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