Effect of dietary levels of lavender oil on Muc2 gene expression in broiler chickens

Document Type : (original research)

Authors

1 Department of Animal Science, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

2 Department of Animal Science, Abhar Branch, Islamic Azad University, Abhar, Iran

Abstract

In this study, the expression of MUC2 gene related to immunity in Arian strain broiler chickens was investigated and its expression changes were investigated by adding different levels of lavender essential oil. The experiment was conducted in the form of a completely randomized design with 5 treatments and 4 repetitions, and each repetition included 25 pieces of chicken. Experimental treatments included: basic diet, basic diet containing 100 mg of probiotic, 150 mg of antibiotic, 200 mg of lavender essential oil and 400 mg of lavender essential oil. In order to investigate the expression of the MUC2 gene, first, total RNA was extracted from the genome part of the intestinal tissue of chickens of different treatments, and after making cDNA from the samples, the expression of the gene was analyzed using Real Time PCR method. The findings were analyzed using the GLM method of SAS statistical software (9.1) and the mean of the treatments were compared using Duncan's test. The results showed that the expression of MUC2 gene in intestinal tissue increased, but there was no significant difference in different levels of treatments. The findings of this study showed that the response to sheep red blood cells, immunoglobulin G and immunoglobulin M was not affected by the treatments and the expression of the MUC2 gene increased in the treated broilers. Therefore, according to the fact that the use of lavender medicinal plant causes the development of intestinal flora and this also causes the increase of mucin secretion in the intestine. Therefore, adding lavender to the diet of broiler chickens due to its probiotic role improves the immune system and prevents diseases such as ascites, and also causes positive effects on the health of the digestive system.

Keywords

Main Subjects

References

  1. Duke, J. and Beckstrom-Stenberg, S.M., 1994. Acceptable levels of flavoring ingredients., In: charalammbous G (Ed). Developments in Food Science. 34: 741-758.
  2. Soltan, M.A., Shewita, R.S. and El-Katcha, M.I., 2008. Effects of dairy anise seeds supplementation on growth performance, immune response, carcass traits and some blood parameters of broiler chickens. Journal of Poultry Science. 7(11): 1078-1088.
  3. Horosova, K., Bujnakova, H. and Kmet, V., 2006. Effect of oregano essential oil on chicken lactobacilli and Coli Journal of Floia Microbiology. 51(4): 278-280.
  4. Jamroz, D., Wiliczkiewicz, A., Wertelecki, T., Orda, J. and Scorupinska, J., 2005. Use of active substances of plant origin in chicken diets based on maize and domestic grains. Journal of Poultry Science. 46(4): 485-493.
  5. NRC. 1994. Nutrient Requirements of Poultry. 9th Ed. Natl. Acad. Sci., Washington DC. 114 p.
  6. Ambrose, T. and Donner, A., 1973. Application of the analysis of variance to hemagglutination titrations. J Immunol Methods. 3(2):165-209. doi: 10.1016/0022-1759(73)90031-8.
  7. Hisako, S., Yamagishi, A., Yoshida, J., Nakano, H. and Hoshino, N., 2005. A Microscopic Model for Helical Twisting Power by the Optical Isomers of an Octahedral Metal Complex. Japanese Journal of Applied Physics. 44(6): 40-67.
  8. Delahunty, C. and Salmon, J.R., 1996. Protein identification using 2D-LC-MS/MS. Methods. 35: 248-255.
  9. Smirnov, A., Perez, R., Romach, E.A., Sklan, D. and Uni, Z., 2005. Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation. American Society for Nutritional Sciences. 135(2): 187-192.
  10. Smirnov, A., Sklan, D. and Uni, Z., 2004. Mucin dynamics in the chick small intestine are altered by starvation. J. Nutr. 134(4):736-742.
  11. Lee, K.W. and Beynen, A.C., 2004. Essential oils in broiler nutrition. International Journal of Poultry Science. 3(1): 738-752.
  12. Gibson, G.R. and Roberfroid, BM., 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition. 125(6): 1401-1412.
  13. Jamroz, D., Wertelecki, T., Houszka, M. and Kamel, C., 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. Journal of Animal Physiology and Animal Nutrition. 90(5-6): 255-268.
  14. Lee, K.W., Everts, H., Kappert, H.J., Frehner, M., Losa, R. and Beynen, A.C., 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science. 44(3): 450-457.
  15. Jamroz, D., Wiliczkiewicz, A., Wertelecki, T., Orda, J. and Scorupinska, J., 2005. Use of active substances of plant origin in chicken diets based on maize and domestic grains. Journal of Poultry Science. 46(4): 485-493.
  16. Jamroz, D. and Kamel, C., 2002. Plant extracts enhance broiler performance. Journal of Animal Science. 80(4): 140-148.
  17. Smirnov, E., Tako, PR. and Uni, Z., 2006. Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydrates. Poultry Science. 85(4): 669-673.
  18. Van der Sluis, M., Melis, H.M., Jonckheerec, N., Marie-Paule, D.A., Bullera, H. and Van Seuningenc, I., 2004. The murine Muc2 mucin gene is transcriptionally regulated by the zinc-finger GATA-4 transcription factor in the intestinal Biochemical and Biophysical Research Communications. 325(3): 952-996.
  19. Ami Azghadi, M., Pilehvar, M., Arshami, J. and Khani, A.M., 2010. Effect of different levels of thyme and cumin extracts on production performance, egg quality and humoral immune response in laying hens. Proceedings of the 4th Congress of Animal Sciences of Iran. Karaj. (In Persian)
Journal of Animal Environment
Volume 14, Issue 2
July 2022
Pages 113-122

Files

Share

How to cite

Statistics