Comparative sterilizing between livebearer and oviparous fishes by oral treatment of 17α-methyl testosterone; case study on guppy (Poecilia reticulata) and convict cichlid (Cichlasoma nigrofasciatum)

Document Type : (original research)

Authors

1 Department of Fisheries, Bandargaz Branch, Islamic Azad University, Bandargaz, Iran

2 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

Abstract

Fish sterilization methods are normally used aiming increase growth rate and avoiding early and undesirable breeding. Sex reversal and sterilization due to steroid hormones are some of the most common methods in culture of ornamental fish. Also, the difference in the sensitivity of livebearers and oviparous fish to hormone treatments has been well discussed previously. Therefore, the present study was conducted to compare the sterilization due to oral treatment of 17-alpha-methyl testosterone (MT) in the livebearer, Poecilia reticulata and the oviparous, Cichlasoma nigrofasciatum. Guppy and Cichlid fries were divided into seven groups and were fed at the first external feeding by commercial diet contains 30, 60, 100, 150, 200, 300 and 0 (control) mg of hormone 17 alpha-methyl Testosterone per kilogram of food respectively, 6 times a day for 40 days. Each treatment was performed with three replications and 40 fish. The hormone was added to the food by the alcohol dry method. After the hormonal treatment period, the gonads of the fish were examined histologically. The results of present study showed that 300 MT hormone per kg of food caused in 97.6% of sterilized individuals in cichlid, which had a significant difference with other treatments (P<0.05). Also, 300 mg of the hormone per kg of food caused 37.7% of sterilized individuals in guppy, which had a significant difference with other treatments (P<0.05). The results showed that there is a direct and significant relationship between increasing the concentration of hormones and the production of sterility. Also, the results showed that cichlid is more sensitivity to the hormone than a guppy. In conclusion, it can be argued that the oral treatment of MT causes sterilized populations, which can avoid early and undesirable breeding. As a result, reducing the fish culture period is useful in terms of time and economic efficiency for fish culture.

Keywords

Main Subjects

References

  1. Ighdari, S., Mousavi Sabet, H. and Hasanalipour, A., 2014. A practical guide to breeding and rearing freshwater ornamental fish. Iranian Fisheries Society and Serva Publications. 156 p. (In Persian)
  2. Pandian, T.J., 2000. Masculinization in Gambusia holbrooki with 17a-methyl testosterone. Aquaculture. 189: 311-319.
  3. Pandian, T.J. and Kirankumar, S., 2003.Recent advances in hormonal induction of sex-reversal in fish. J. of Appl. Aquaculture. 13(3): 205-230.
  4. Piferrer, F. and Lim, L.C., 1997. Application of sex reversal technology in ornamental fish
    Aquar. Sci. and Cons. 1: 113-118.
  5. Galvez, J.I., Morrison, J.R. and Phelps, R.P., 1996. Efficacy of Trenbolone Acetate in Sex Inversion of the Blue Tilapia Oreochromis aureus. Journal of the Worl. Aquaculture Soc. 27(4): 483-486. 
  6. Farahmand, H., 1993. Causing sex change and sterility in common carp by 17-alpha-methyltestosterone hormone. Master thesis. Faculty of Natural Resources, Tarbiat Modares University. 102 p. (In Persian)
  7. Hatfi, M., 1998. Sex change in guppy fish (Poecilia reticulata) using methyltestosterone and ethinylestradiol hormones. master thesis. Islamic azad university Lahijan branch. Iran. (In Persian)
  8. Amini Charmahini, M., 2018. Investigating the possibility of impregnation of guppy fish by the hormone 17 alpha-methyltestosterone. Master thesis. Faculty of Natural Resources, University of Tehran. 98 p. (In Persian)
  9. Alamdoust, A., 2015. Investigating the possibility of impregnating blue hop fish (Sciaenochromis ahli) using 17 alpha-methyltestosterone. master thesis. Faculty of Natural Resources, University of Tehran. Iran. (In Persian)
  10. Mousavi-Sabet, H., Langroudi, H.F. and RohaniRad, M., 2012. Sex reversal, mortality rate and growth of guppy (Poecilia reticulata) affected by 17-alpha methyltestosterone. Poeciliid Res. 2(1): 1-8.
  11. Piferrer, F., 2001. Endocrine sex control strategies for feminization of teleost fish. Aquaculture. 197(1-4): 229-281.
  12. Mousavi-Sabet, H., 2011. The effect of 17-alpha methyl testosterone on masculinization,
    mortality rate and growth in convict cichlid (Cichlasoma nigrofasciatum). World of Fish and Marine Sci. 3(5): 422-426.
  13. Mousavi-Sabet, H. and Ghasemnezhad, H., 2013. Masculinization, mortality and growth rates of swordtail Xiphophorus hellerii (Poeciliidae) affected by methyltestosterone. Poeciliid Res. 3(1): 7-13.
  14. Marjani, M., Jamili, Sh., Mostafavi, P.G., Ramin, M. and Mashinchian, A., 2009. Influence of 17- alpha methyl testosterone on masculinization and growth in tilapia (Oreochromis mossambicus). J. of Fisheries and Aqua. Sci. 4(1): 71-74.
  15. George, T. and Pandian, T., 1996. Hormonal induction of sex reversal and progeny testing in the zebra cichlid Cichlasoma nigrofasciatum. J. Exp. Zool. 275(5): 374-382.
  16. Blazquez, M., 2001. Critical period of androgen‐inducible sex differentiation in a teleost fish, the European sea bass. Journal of Fish Biol. 58(2): 342-358.
  17. Lee, P., King, H. and Pankhurst, N., 2004. Preliminary Assessment of Sex Inversion of Farmed Atlantic Salmon by Dietary and Immersion Androgen Treatments. North American J. of Aquaculture. 66(1): 1-7. 
  18. Khiabani, A., Anvarifar, H. and Mousavi-Sabet, H., 2016. Effect of dietary administration of methyltestosterone and vitamin C on the sex reversal and survival of Xiphophorus maculatus (Cyprinodontiformes: Poeciliidae). Poeciliid Res. 6(1): 16-24.
  19. Mousavi-Sabet, , Zamini, A., Vahabzadeh Rudsari, H. and Moradkhani, Z., 2018. A comparative study of the death rate caused by oral administration of 17-alpha-methyltestosterone hormone in guppy fish (Poecilia reticulata) and zebra cichlid (Cichlasoma nigrofasciatum). Veterinary Journal of Islamic Azad University. 9: 45-49. (In Persian)
Journal of Animal Environment
Volume 14, Issue 1
Spring issue
May 2022
Pages 205-208

Files

Share

How to cite

Statistics