The Effect of adding rosemary aqueous extract in to extender on cryopreservation of ram semen

Document Type : (original research)

Authors

Department of Animal Science, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran

10.22034/AEJ.2021.276096.2477

Abstract

One of the freezing semen disadvantages is increasing of free radicals leading to enhanced sperm membrane peroxidation then consequently causes lowered semen fertility after thawing. Some plants bearing high level of natural antioxidants can help to decrease semen peroxidation. The objective of the present study is to evaluate the ability of rosemary extract to protect ram sperm from freeze-thaw damages. In this study, ejaculates were collected from 4 Zandi rams. Semen samples were pooled after each sampling. Experimental treatment included five levels of rosemary aqueous extract (0, 2.5, 5, 7.5 and 10 percent). Then samples were frozen and transferred to liquid nitrogen tanks for three weeks. After thawing sperm quality parameters including total and progressive motility, abnormalities, sperm membrane integrity and MDA production were evaluated. The results showed that rosemary extract significantly improved total and progressive motility, sperm membrane integrity and viability, and decreased sperm abnormalities and MDA production at levels of 5 and 7.5 percent (p<0.05). According to the obtained data, adding specific levels of rosemary extract in the diluent will have a protective effect in the freezing-thawing process of Zandi rams sperm.

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References

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Journal of Animal Environment
Volume 14, Issue 1
Spring issue
May 2022
Pages 49-56

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