The effect of different levels of Rosa canina hydroalcoholic extract on the development of hypopharyngeal glands, growth parameters and honey production of honey bee colonies (Apis mellifera)

Document Type : (original research)

Authors

1 Department of Animal Sciences, Faculty of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Animal Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

3 Excir Faravaran Sabalan Company, Science and Technology Park, Ardabil, Iran

10.22034/aej.2022.336670.2786

Abstract

Today, for the preservation and survival of honey bee colonies, special attention has been paid to the nutrition of colonies with extracts of medicinal plants. In this experiment, the effect of different levels of Rosa canina hydroalcoholic extract on population, queen egg laying, brood rearing, honey production and development of the hypopharyngeal glands of honey bee colonies (Apis mellifera) were studied during summer in an apiary in Ardabil province. At the beginning of the experiment, the colonies were homogenized according to the population, food storage and queens of the same age. The colonies were fed with control treatment and different concentrations of Rosa canina extract (2, 4 and 8% of the extract) during1 month. Brood rearing and queen egg laying were measured by Lattice frame (size of the squares are 5×5 cm2). The population of adult honey bee was measured by frame and 30 days after feeding the colonies. To investigate development of the hypopharyngeal glands, length and width of 10 acini from different parts of the gland for age 3, 6, 9, 12 and 15 days were measured using a stereomicroscope and by software (T Capture).This experiment was performed in a completely randomized design with four replications and four treatments. In this study, treatment of 2% Rosa canina extract had a significant effect on brood rearing, but in queen egg laying, population and honey production, although it had a better effect numerically, but this effect was not statistically significant. In the growth of hypopharyngeal glands, the highest growth of acini level at 3 days (0.0432 mm2) for honey bees fed with 4% Rosa canina extract and at 9 and 12 days (respectively 0.0763 and 0.0722 mm2) It belonged to the colonies fed with 2% Rosa canina extract (P<0.05). In general, it can be said that the use of 2% of Rosa canina hydroalcoholic extract has been effective in improving the performance of honey bee colonies. It is also recommended for beekeepers to use Rosa canina hydroalcoholic extract as a nutritional supplement.

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References

  1. Demir, F. and Özcan, M., 2001. Chemical and technological properties of rose (Rosa canina L.) fruits grown wild in Turkey. Journal of food engineering. 47(4): 333-336.
  2. Mattila, H. and Otis, G., 2007. Manipulating pollen supply in honey bee colonies during the fall does not affect the performance of winter bees. The Canadian Entomologist. 139(4): 554-563.
  3. De Grandi-Hoffman, G., Chen, Y., Huang, E. and Huang, M.H., 2010. The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.). Journal of Insect Physiology. 56(9): 1184-1191.
  4. Corby-Harris, V. and Snyder, L.A., 2018. Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers.  Vis. Exp.139: e58261.
  5. Corby-Harris, V., Meador, C.A., Snyder, L.A., Schwan, M.R., Maes, P., Jones, B.M., Walton, A. and Anderson, K.E., 2015. Transcriptional, translational, and physiological signatures of undernourished honey bees (Apis mellifera) suggest a role for hormonal factors in hypopharyngeal gland degradation. J. Insect Physiol. 85: 65-75.
  6. Patruica, S., Mot, D. and­ Popovici, D., 2017. The effect of using medicinal plant extracts upon the health of bee colonies. Romanian Biotechnological Letters. 6(22): 13182-13185.
  7. Pohorecka, K., 2004. Effect of standardized plant herb extracts on general condition of the honey bee. Bulletin of the Veterinary Institute in Pulawy. 48(4): 415-419.
  8. Marghitaş, L.A., Bobiş, O. and Tofalvi, M., 2010. The effect of Plant supplements on the development of artificially weaken bee families. Scientific Papers Animal Science and Biotechnologies. 43(1): 402-406.
  9. Patruica, S., Dunea, I.B., Jivan, A., Jivan, A. and Stroe, A., 2011. Research on the Influence of Apiary Biostimulators on Bee Families Development in spring. Scientific Papers: Animal Science and Biotechnologies. 44(2): 267-270.
  10. Al-Ghamdi, A.A., Abou -Shaara, H.F. and Ansari, M.J., 2021. Effects of sugar feeding supplemented with three plant extracts on some parameters of honey bee colonies. Saudi Journal of Biological Sciences. 28(4): 2076-2082.
  11. Zhang, Q.W., Lin, LG. and Ye, W.C., 2018. Techniques for extraction and isolation of natural products: a comprehensive Chinese Medicine. 13: 20 p.
  12. Sharma, V.P. and Kumar, N.R., Changes in honeybee behaviour and biology under the influence of cellphone radiations. Current Science. 98(10): 1376-1378.
  13. DeGrandi-Hoffmanh, G., Wardell, G., Ahumada-Segura, F., Rinderer, T., Danka, R. and Pettis, J., 2008. Comparisons of pollen substitute diets for honey bees: consumption rates by colonies and effects on brood and adult populations. Journal of Apicultural Research and Bee World. 47: 265-270.
  14. Mohebodini, H., Tahmasbi, Gh.H., Dastar, B., Jafari Ahangari, Y. and Zerehdaran, S., 2013. Effect of dietary thiamin on growth of the Iranian honey bee colonies COLONIES (Apis mellifera meda) in different seasons. Agriculture and Forestry. 59: 119-126.
  15. Zarin, F., 2000. Investigating the level of homozygosity of sexual alleles and its correlation with honey production in honey bee colonies of Tehran, Isfahan, Central, and Qazvin provinces. Master's thesis of honey bee, Imam Khomeini Higher Education Center. (In Persian)
  16. Al-Ghamdi, A.A., Al-Khaibari, A.M. and Omar, M.O., 2011. Consumption rate of some proteinic diets affecting hypopharyngeal glands development in honeybee workers. Saudi Journal of Biological Sciences. 18: 73-77.
  17. Mohebodini, H.,Tahmasbi, Gh., Dastar, B., Jafari Ahangari, Y. and Zerehdaran, S., 2016. The hypopharyngeal glands development in Iranian honey bee workers (Apis mellifera meda) fed with different levels of thiamine. Animal Science Journal. 29(110): 203-212. (In Persian)
  18. SAS Institute INC. 2013. SAS STATS users Guide. Version 9.1.3, SAS Institute Inc. Cary, N.C
  19. Melo, I.L.P. and Almeida-Muradian, L.B., 2010. Stability of antioxidants vitamins in bee pollen samples. Quim. Nova. 33(3): 514-518.
  20. Jovanovic, N.M., Glavinic, U., Delic, B., Vejnovic, B., Aleksic, N., Mladjan, V. and Stanimirovic, Z., 2021. Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance. Preventive Veterinary Medicine. 190: 105322.
  21. Ahmadi, A., Nehzati-paghale, Gh., Amiri Zandi, M. and Abbasi, S., 2014.  Effect of different levels of vitamin C on queen laying rate and body protein percentage of worker in honey bee colonies (Apis mellifera L.). Iranian Journal of Animal Science. 45(1): 37-42. (In Persian)
  22. Zahra, A. and Talal, M., 2008. Impact of pollen supplements and vitamins on the development of hypopharyngeal glands and brood area in honey bees. Journal of Apicultural Science. 52: 5-12.
  23. Andi, M.A. and Ahmadi, A.Y., 2014. Influence of vitamin C in sugar syrup on brood area, colony population, body weight and protein in honey bees. International Journal of Biosciences. 4(6): 32-36.
  24. Majewska, B.M. and Jasinski, Z., 2005. Effect of early supplemental feeding honeybee colonies with a substitute of bee bread made of drone brood candy, glucose and honey on colony strencth. Journal of Apicultural Science. 41: 1-49.
  25. Hrassnigg, N. and Crailsheim, K., 1998. The influence of brood on the pollen consumption of worker bees (Apis mellifera L.). Journal of Insect Physiology. 44: 393-404.
  26. Salama, S.A., 2020. Effect of some food additives on honey bee hypopharyngeal glands (Apis mellifera) in the south of Saudi Arabia. World Wide Journal of Multidisciplinary Research and Development. 6(11): 53-60.
  27. Cheng, C., Zhang, X., Shen, G. and Liu, A., 1993. The effect of feeding syrup with vitamin E on the development of the pharyngeal gland of the worker bee. 33rd International Apicultural Congress of Apimondia, 20-26 September 1993.Beijing, China.
  28. Zheng, B., Wu, Z. and & Xu, B., 2014. The effects of dietary protein levels on the population growth, performance, and physiology of honey bee workers during early spring. Journal of insect science (Online). 14: 191.
  29. Atallah, M.A., Mofttah, E.A., Eahbah, H.M., Mohamed, A.A. and Eyssa, N.A., 1995. Effect of protein feeding on the development of hypopharyngeal gland of two races of honeybee and the chemical composition of royal jelly. 6th Nat. Conf. of Pest. & Dis. of Veg. &Fruits in Egypt. 88-100.
  30. Feng, M., Fang, Y. and Li, J., 2009. Proteomic analysis of honeybee worker (Apis mellifera) hypopharyngeal gland development. BMC Genomics. 10: 645-657.
  31. Almeida-Muradian, L.B., Pamplona, L.C., Coimbra, S. and Barth, O.M., 2005. Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis. Journal of Food Composition and Analysis. 18: 105-111.
Journal of Animal Environment
Volume 15, Issue 1
June 2023
Pages 237-244

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