Evaluation of expression profile of intestinal cells infected with Cryptosporidium parvum using bioinformatics

Document Type : (original research)

Authors

1 Department of Parasitology, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Parasitology, Shahr-e-kord Branch, Islamic Azad University, Shahr-e-kord, Iran

3 Department of Parasitology, Tehran University, Tehran, Iran

10.22034/aej.2022.316508.2799

Abstract

Pathogens affect host cellular mechanisms in order to impair the capabilities of the host cell in the path of infection.Cryptosporidium parvum in people with AIDS and people with weakness of the immune system creates severe bile and acute diarrhea, and is known as an important intestinal pathogen in humans and some mammals and rows with Salmonella, Shigella and Enterotoxic genetic strains of Escherichiacoli It takes. From the point of view of cellular genetics, it should be noted that human intestinal cells due to infection with Cryptosporidium parvum undergo major changes in host-parasite interactions, especially changes in the expression of relevant genes. The aim of this study was to identify the effects of altering the expression profile of genes in HCT-8 cells infected with Cryptosporidium parvum on host-parasite interaction. In this study, an estimate of the number of genes involved in biological processes and in the process of intestinal cell infection with Cryptosporidium parvum was obtained. In order to carry out the studies, the data recorded in the GEO database on the NCBI website, which was placed by researchers at the University of Houston, Texas, USA, were used. Raw data were read, pre-processed and processed using various software and online databases. Studies have shown that the expression of genes involved in HCT-8 cell line infection is affected by Cryptosporidium parvum, and that some genes are over-expressed and some are under-expressed. The results of this study show that the most genes involved in biological processes are related to cell-parasite communication and the least genes are involved in cell death (apoptosis) and DNA replication. The results of this study show that different genes under the influence of infection change in expression, each of which can activate different factors and different pathways in the direction of infection and be considered as future drug and vaccine targets.

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References

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Journal of Animal Environment
Volume 15, Issue 1
June 2023
Pages 87-92

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