The inhibitory effect of methanolic, acetone, and aqueous extracts of razor clam (Solen vagina) on the growth of Vibrio harveyi bacteria in post-larvae of Vannamei shrimp (Litopenaeus vannamei)

Document Type : (original research)

Authors

1 Department of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran

2 Department of Fisheries, Marine and Fisheries Technologies Research Center, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran

3 Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization ,Tehran, Iran

4 Iranian Fisheries Science Research Institute, Agriculture Research Education and Organization, Tehran, Iran

10.22034/aej.2022.374111.2908

Abstract

This study was aimed to compare the halo diameter (mm) caused by the lack of bacteria growth (Vibrio harvey) in the post-larvae of Vannamei shrimp (Litopenaeus vannamei) under the influence of methanolic, acetone, and aqueous extracts (ppm) of razor clam (Solen vagina). Based on the obtained results, the methanolic extract stopped the growth of Vibrio harvey bacteria at 450 ppm. Although the use of this extract stopped the growth of bacteria at a concentration of 450 ppm, the diameter of the created halo was smaller compared to the halo of non-growth caused by antibiotics. Acetone extract prevented the growth of Vibrio harvey bacteria at 250 ppm and higher concentrations and created a lack of growth halo with a diameter of 2.8 cm. Also, the aqueous extract did not have any inhibitory effect. The results of the mean comparison test showed that there was a significant difference between different concentrations of methanolic and acetone extracts (P<0/05). Also, significant differences were observed between the same effective concentrations of different treatments (250, 300, 350, 400 and 450 ppm) (P<0/05). Therefore, methanolic and acetone extracts of S. vagina can be used as a bioactive substance to prevent vibriosis in Litopenaeus vannamei. However, more studies are suggested to investigate other extracts of this species and their effects.

Keywords

Main Subjects

References

  1. Vandenberghe, J., Verdonck, L., Robles-Arozarena, R., Rivera, G., Bolland, A. and Balladares, M., 1999. Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. Applied and Environmental Microbiology. 65(6): 2592-2597.
  2. Lavilla-Pitogo, C.R., Leaño, E.M. and Paner, M.G., 1998. Mortalities of pond-cultured juvenile shrimp, Penaeus monodon, associated with dominance of luminescent vibrios in the rearing environment. Aquaculture. 164(1-4): 337-349.
  3. Chrisolite, B., Thiyagarajan, S., Alavandi, S., Abhilash, E., Kalaimani, N. and Vijayan, K., 2008. Distribution of luminescent Vibrio harveyi and their bacteriophages in a commercial shrimp hatchery in South India. Aquaculture. 275(1-4): 13-19.
  4. Uma, A., Meena, S., Saravanabava, K. and Muralimanohar, B., Identification of bacterial pathogens infecting Penaeus monodon, tiger shrimp by 16S rDNA amplification and sequencing.
  5. Bhakuni, D.S. and Rawat, D.S., 2006. Bioactive marine natural products: Springer Science & Business Media;.
  6. Buchanan, J., 2004. Missing links? Problem drug use and social exclusion. Probation Journal. 51(4): 387-397.
  7. Anand, T.P., Rajaganapathi, J. and Edward, J., 1997. Antibacterial activity of marine molluscs from Portonovo region.
  8. Kiran, N., Siddiqui, G., Khan, A., Ibrar, K. and Tushar, P., 2014. Extraction and screening of bioactive compounds with antimicrobial properties from selected species of mollusk and crustacean. Journal of Clinical & Cellular Immunology. 5(1): 1-5.
  9. Haug, T., Stensvåg, K., Olsen, Ø.M., Sandsdalen, E. and Styrvold, O.B., 2004. Antibacterial activities in various tissues of the horse mussel, Modiolus modiolus. Journal of Invertebrate pathology. 85(2): 112-119.
  10. Manilal, A., Selvin, J., Sugathan, S. and Panikkar, M., 2012. Evaluation of therapeutic efficacy of Indian green alga, Acrosiphonia orientalis (J. agardh) in the treatment of vibriosis in Penaeus monodon. Thalassas An International Journal of Marine Sciences. 28(1): 33-46.
  11. Huynh, T.G., Yeh, S.T., Lin, Y.C., Shyu, J.F., Chen, L.L. and Chen, J.C., 2011. White shrimp Litopenaeus vannamei immersed in seawater containing Sargassum hemiphyllum chinense powder and its extract showed increased immunity and resistance against Vibrio alginolyticus and white spot syndrome virus. Fish & Shellfish Immunology. 31(2): 286-293.
  12. Eswar, A., Ramamoorthy, K., Mohanraj, M., Gokulakrishnan, S. and Sankar, G., 2014. In-vitro antibacterial activity and Brine Shrimp Lethality Test on selected three marine Mollusks from Velar Estuary, Parangipettai. International Journal of Current Research. 6: 9075-9078.
  13. Hoa, N.D., Wouters, R., Wille, M., Thanh, V., Dong, T.K. and Van Hao, N., 2009. A fresh-food maturation diet with an adequate HUFA composition for broodstock nutrition studies in black tiger shrimp Penaeus monodon (Fabricius, 1798). Aquaculture. 297(1-4): 116-121.
  14. Torpee, S., Kantachote, D., Rattanachuay, P., Chiayvareesajja, S. and Tantirungkij, M., 2021. Dietary supplementation with probiotic Rhodobacter sphaeroides SS15 extract to control acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus in cultivated white shrimp. Journal of Invertebrate Pathology. 186: 107585.
  15. Karnjana, K., Soowannayan, C. and Wongprasert, K., 2021. Ethanolic extract of red seaweed Gracilaria fisheri and furanone eradicate Vibrio harveyi and Vibrio parahaemolyticus biofilms and ameliorate the bacterial infection 2019 in shrimp. Fish & Shellfish Immunology. 88: 91-101.
  16. Dhinakaran, D.I. and Lipton, A., 2012. Antimicrobial potential of the marine sponge Sigmadocia pumila from the south eastern region of India. World J Fish Mar Sci. 4: 344-348.
  17. Parvekar, P., Palaskar, J., Metgud, S., Maria, R. and Dutta, S., 2020. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus. Biomaterial investigations in dentistry. 7(1): 105-109.
  18. Dorrington, T. and Gomez-Chiarri, M., 2008. Antimicrobial peptides for use in oyster aquaculture: effect on pathogens, commensals, and eukaryotic expression systems. Journal of Shellfish Research. 27(2): 365-373.
  19. Bulet, P., Stöcklin, R. and Menin, L., 2004. Anti‐microbial peptides: from invertebrates to vertebrates. Immunological reviews. 198(1): 169-184.
  20. Hubert, F., Noël, T. and Roch, P., 1996. A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis). European Journal of Biochemistry. 240(1): 302-306.
  21. Bartlett, T.C., Cuthbertson, B.J., Shepard, E.F., Chapman, R.W., Gross, P.S. and Warr, G.W., 2002. Crustins, homologues of an 11.5-kDa antibacterial peptide, from two species of penaeid shrimp, Litopenaeus vannamei and Litopenaeus setiferus. Marine Biotechnology. 4(3): 278-293.
  22. Chellaram, C., Gnanambal, and Edward, J., 2004. Antibacterial activity of the winged oyster Pteria chinensis (Pterioida: Pteridae).
  23. Wouters, R., Lavens, P., Nieto, J. and Sorgeloos, P., 2001. Penaeid shrimp broodstock nutrition: an updated review on research and development. Aquaculture. 2(1-2): 1-21.
  24. Payam, B., Soltani, M., Shamsaie Mehrgan, M., Rajabi Islami, H. and Nazemi, M., 2021. Antibacterial activity of sea cucumber (Holothuria leucospilota) extracts on Lactococcus garvieae and Aeromonas hydrophila. Journal of Animal Environment. 13(3): 307-314. (In Persian)
  25. Azizi, R., Motallebi, A., Nazemi, M., Sharifruhani, M. and Afsharnasab, M., 2020. Survey of antibacterial Properties of Extracts of Green Algae Ulva fasciata collected from Persian Gulf. Journal of Animal Environment. 12(3): 469-474. (In Persian)
  26. Kanjana, K., Radtanatip, T., Asuvapongpatana, S., Withyachumnarnkul, B. and Wongprasert, K., 2011. Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon. Fish & shellfish immunology. 30(1): 389-396.
  27. Sirirustananun, N., Chen, J.C., Lin, Y., Yeh, S.T., Liou, C.H. and Chen, L.L., 2011. Dietary administration of a Gracilaria tenuistipitata extract enhances the immune response and resistance against Vibrio alginolyticus and white spot syndrome virus in the white shrimp Litopenaeus vannamei. Fish & shellfish immunology. 31(6): 848-855.
  28. Eidi Ghaleghazi, F., Noori, A. and Hosseinifar, S.H., 2021. The effects of dietary supplementation of Sargassum ilicifolium hot-water extract on some haemolymph physiological parameters of whiteleg shrimp, Penaeus vannamei. Aquaculture Sciences. 9(1): 1-14.
  29. Esquer-Miranda, E., Nieves-Soto, M., Rivas-Vega, M.E., Miranda-Baeza, A. and Pi, P., 2016. Effects of methanolic macroalgae extracts from Caulerpa sertularioides and Ulva lactuca on Litopenaeus vannamei survival in the presence of Vibrio bacteria. Fish & shellfish immunology. 51: 346-350.
  30. Huang, H.T., Lee, P.T., Liao, Z.H., Chen, H.Y. and Nan, F.H., 2020. Effects of Phyllanthus amarus extract on nonspecific immune responses, growth, and resistance to Vibrio alginolyticus in white shrimp Litopenaeus vannamei. Fish & Shellfish Immunology. 107: 1-8.
  31. Otero‐Gonzáiez, A.J., Magalhaes, B.S., Garcia‐Villarino, M., López‐Abarrategui, C., Sousa, D.A. and Dias, S.C., 2010. Antimicrobial peptides from marine invertebrates as a new frontier for microbial infection The FASEB journal. 24(5): 1320-1334.
Journal of Animal Environment
Volume 15, Issue 3
December 2023
Pages 169-174

Files

Share

How to cite

Statistics