Document Type : (original research)
Authors
1
Department of Health, Aquatic Animal Health and Disease, Science and Research Branch, Islamic Azad University, Tehran, Iran
2
Department of animal science, University college of agriculture and national resources, University of Tehran, Karaj, Iran.
3
Department of Genomics and Genetic Engineering, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
4
Department of Food Hygiene, Science and Research Unit, Islamic Azad University, Tehran, Iran
10.22034/aej.2021.280194.2496
Abstract
Infectious pancreatic necrosis (IPN) is a significant viral disease in the aquaculture industry that sometimes leads to widespread mortality in fish despite the lack of clinical signs, so the use of prevention methods including Vaccination is very important in this disease. In this study, the DNA template and template used to amplify the VP2 gene of PCR product from an Iranian IPNV isolate (IR-IPNV). NZ9000 L. lactis strain was also used as host for cloning and as host for expression. The results showed that cloning and gene expression were well done and after the use of lactobacillus containing genes in the patient's fishes, it increased the antibody level and the viral infection decreased significantly. In this study, for the first time, L. lactis has been used to clone a candidate gene from an infectious pathogen of fish for the production of recombinant live vaccine vaccine. It seems that this expression system can be used to deliver an oral vaccine, Potential is considered as a new platform for the development of oral vaccines in fish.
Keywords
Main Subjects