Surveying the structure and genetic diversity of red foxes in the North East of Iran, based on cytochrome b gene

Document Type : Genetic

Authors

1 Islamic Azad University, Science and Research, Faculty of Environment and Energy, Department of habitats and biodiversity

2 Gorgan University of Agricultural Sciences and Natural Resources, Iran

3 Department of Environmental Science, Faculty of Natural Resources, the University of Guilan, Rasht, Iran

4 Department of Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box:49138-15739, Gorgan, Iran

Abstract

To study the genetic structure of red foxes in North East of Iran, 25 tissue samples were collected from three provinces (Razavi Khorasan, North Khorasan and Golestan). After DNA extraction, mitochondrial cyt-b gene was amplified by primers using polymerase chain reaction (PCR); then 1098 base pairs of this gene were edited for each sample after purification and sequencing processes. Then by analyzing of this partial sequence of the samples: polymorphism sites, nucleotide and haplotype diversity, the amount of genetic differentiation (using Fst index), Demographic history (using Harpending's raggedness index, Tajima and Fu Fs test) were calculated and phylogenetic tree and haplotype network were drawn by Baysian and Median joining logic in order. The results showed that there were 13 different haplotypes and 22 polymorphic sites between the samples and haplotype diversity was equal to 0.88. Fst (P<0.05) index was also equal to 0.6382 which due to this index and the results of the analysis of molecular variance (AMOVA), there is limited gene flow between populations and populations are distinct with each other to high extent. Phylogenetic tree showed that there were independent genetic structure for considered populations and drawn haplotype network was also confirmed this issue. Accordingly, it could be concluded that the red foxes would have had a complicated evolutionary history and distribution pattern of this species in the North East of Iran would be the result of a long process.

Keywords

Journal of Animal Environment
Volume 8, Issue 4
January 2017
Pages 25-34

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