The effect of eyestalk ablation and hormone injection on Reproductive performance and physiological stress in Litopenaeus vannamei

Document Type : Biology(Animal)

Authors

1 Department of Fisheries, Faculty of Agricultural Sciences and Natural Resources, University of Gonbad-e Qabus, POBox: 163

2 Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, POBox: 487-49175

3 Research Institute of Fisheries Science countries, Organization of research, education and extension, Tehran, POBox: 6116- 14155

Abstract

Eyestalk ablation is the most common procedure to induce gonadic maturation in commercial hatcheries of penaeid shrimp. This study was conducted in order to investigate the potential of using Ovaprim (gonadotropin and dopamine antagonist) hormone to induce ovarian maturation on Litopenaeus vannamei. The female broodstocks were divided into 4 treatments as follows: 1) Eyestalk ablation; 2 and 3) Ovaprim injections at 500 and 1000 ng/g body weight (BW), respectively; 4) sterile vehicle solution injection (control). Reproductive parameters were evaluated such as the number of eggs, oocyte diameters and gonadosomatic indices and number of nauplii. The results of the analysis of reproductive factors showed that there was significant difference between experimental treatments (p< 0.05). The highest average number of egg and nauplii (83100± 16993 and 56132± 5180 respectively) was obtained in eyestalk ablation treatment. The weakest reproductive performance was observed in the sterile vehicle solution injection treatment (control). Hemolymph changs to physiological stress (eyestalk ablation and injection treatments) was investigated. The results showed that humoral cell number was no significant statistical differences between eyestalk ablation and injection treatments (p> 0.05). Metabolic response (protein, glucose, lactate and cortisol) was significantly different between experimental treatments at the time 0 and 45 minutes (p< 0.05).  The both time, metabolic parameters increase in the eyestalk ablation treatment and was reduced injection treatment.

Keywords

Journal of Animal Environment
Volume 8, Issue 4
January 2017
Pages 219-226

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