Using analog implant LHRH methods and environmental factors (temperature and photoperiod) to out-of season goldfish culture (Carassius auratus)

Document Type : Physiology (Animal)

Authors

1 Departeman of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Iran

2 Department of Fisherie Sciences, University of Kurdistan, P. O. BOX 416, Sanandaj, Iran

3 Inland Water Aquatics Stocks Reserch Center, Gorgan, Iran

4 Department of Fisheries, University of Guilan, Someh sara, Iran

Abstract

  The present research aims to evaluate the effects of different methods of LHRHa implants and injections combined with environmental factors changes (temperature and photoperiod) on some biological properties of semen (spermatocrit percent, Spermatization  volume) and treatment to ovulation, hatch percent, gonadosomatic index (female) and changes in steroid hormones in males and female goldfish in out-of season reproduction. Fish were included in 4 treatments (injection of normal saline with 0.7%, EVAc   20 micrograms / kg body weight LHRHa with 40 micrograms/ kg body weight Metoclopramide, cholesterol pellets containing 20 micrograms / kg body weight LHRHa with 40 mg / kg body weight Metoclopramide and 100 mg / kg LHRH with 40 mg / kg body weight were Metoclopramide). The results show that time to ovulation between different treatments, were significantly different (P <0.05), so that minimum injection time of ovulation was observed in LHRHa and highest one was recorded in cholesterol pellet. The EVAc and cholesterol accounted for highest and lowest percentage of hatched eggs respectively (P <0.05). Their longest period of sperm motility was attributed to EVAC treatment and there was no significant difference between the treatment groups (P> 0.05). Spermatization volume in different treatments was varied in significant manner (P <0.05) so that the highest and the lowest was observed in the treatments EVAc and control. The highest spermatocrit percentage was in EVAc treatment (P <0.05). Changes in levels of testosterone in EVAc and LHRHa injection showed significant difference compared to other treatments (P≤0.05) and at days 7, 14 and 21 days after treatment this hormone was decreased gradually in blood serum of male goldfish broodstock. The results indicated that in female’s the levels of 17α Digydroxy Progestrin and 17 β Estradiol were significantly different among treatments (P<0.05), in EVAc was higher than other treatments. At days 14 and 21 days after treatment these hormones were increased.

Keywords

Journal of Animal Environment
Volume 9, Issue 1
April 2017
Pages 223-230

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