Effects of 4-nonylphenol on production of plasma vitellogenine and changes Steroid hormones in koi carp (Cyprinus carpio carpio)

Document Type : Physiology (Animal)

Authors

1 Asosiated Iranian Fisheries Science Research Institute, Agriculture Research,Education and Extension Organization Institute

2 Department of Fisheries, Islamic Azad University, Science and Research Branch, Tehran, POBox: 1477893855

3 Aquaculture Department, Faculty of Veterinary Medicine, University of Tehran, Iran, POBox: 1419963111

Abstract

Estrogenic   effect  of   4 - nonylphenol  on   plasma  vitellogenin  and  sex  steroid  hormones  (17 – β-estradiol ,testosterone and  progesterone ) were studied in  immature  Koi  carp .  In  order to,  fish  for
 over three weeks Received  Intraperitoneal  injection  of  10 ,  50  and  100  microgram  per  gram  of  4  -nonylphenol  and  2  microgram  per  gram  of   17-  β-estradiol  per  body  weight .  Fish  samples
 were taken on days 0 and 22. The concentration of  sex hormones in plasma  measured by radioimmunoassay
 and  Plasma  vitellogenin levels were  measured  by ELISAThe results showed that amount of vitellogenin
  protein and estradiol hormne one  at  concentration  50 micrograms of  4- nonylphenol  (51/66± 21/3)
 and 2 micrograms of  17 β-estradiol (58±6/02) in males and females increased  significantly compared with
 the control group(P <0.05).But the changes in  vitellogenin  and 17- β-estradiol at a concentration of 100
 micrograms nonylphenol showed  significantly reduced compared with  concentrations of  10  and  50  
 micrograms  of   nonylphenol  (P <0.05). In  the case of  progesterone in all  the groups that were treated with
 nonylphenol ,the effect was not showed statistically significant,and only  at concentrations of  2 micrograms
 17-β-estradiol showed significant decrease(P> 0.05),Also testosterone levels,except in group 2  microgram
 17-β-estradiol treated group had no significan  difference(P >0.05). These  results  indicate harmful effects of  4-
 nonylphenol using  hormonal  markers  and  assess  the  susceptibility  of  immature  Koi  Carp  are against these
 compounds .

Keywords

Journal of Animal Environment
Volume 9, Issue 3
September 2017
Pages 185-194

Files

Share

How to cite

Statistics